Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CD71/Transferrin Receptor Rabbit pAb (A5865)

Publications (34) Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - CD71/Transferrin Receptor Rabbit pAb (A5865)

Western blot analysis of extracts of various cell lines, using CD71/Transferrin Receptor antibody (A5865) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunohistochemistry of paraffin-embedded rat kidney using CD71/Transferrin Receptor antibody (A5865) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunohistochemistry of paraffin-embedded human liver cancer using CD71/Transferrin Receptor antibody (A5865) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunohistochemistry of paraffin-embedded human colon carcinoma using CD71/Transferrin Receptor antibody (A5865) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunohistochemistry of paraffin-embedded human placenta using CD71/Transferrin Receptor antibody (A5865) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunofluorescence analysis of PC-12 cells using CD71/Transferrin Receptor Rabbit pAb (A5865) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameCD71/Transferrin Receptor Rabbit pAb
Catalog No.A5865
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified.

Immunogen information

ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 100-250 of human CD71/Transferrin Receptor (NP_003225.2).
SequenceRLAGTESPVREEPGEDFPAARRLYWDDLKRKLSEKLDSTDFTGTIKLLNENSYVPREAGSQKDENLALYVENQFREFKLSKVWRDQHFVKIQVKDSAQNSVIIVDKNGRLVYLVENPGGYVAYSKAATVTGKLVHANFGTKKDFEDLYTPV
Gene ID7037
Swiss protP02786
SynonymsTFRC; CD71; IMD46; T9; TFR; TFR1; TR; TRFR; p90
Calculated MW84kDa
Observed MW100KDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesHeLa, PC-3
Cellular locationCell membrane, Melanosome, Secreted, Single-pass type II membrane protein
Customer validation

WB(Homo sapiens, Rattus norvegicus, Mus musculus, Gallus gallus, Danio rerio)

IF(Rattus norvegicus)

IF(Rattus norvegicus)

IHC(Mus musculus)

IHC(Mus musculus, Homo sapiens)

WB、IF(Mus musculus)

Research Area

CD71/Transferrin Receptor Rabbit pAb images

ABclonal:Western blot - CD71/Transferrin Receptor Rabbit pAb (A5865)}

Western blot - CD71/Transferrin Receptor Rabbit pAb (A5865)

Western blot analysis of extracts of various cell lines, using CD71/Transferrin Receptor antibody (A5865) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)}

Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunohistochemistry of paraffin-embedded rat kidney using CD71/Transferrin Receptor antibody (A5865) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)}

Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunohistochemistry of paraffin-embedded human liver cancer using CD71/Transferrin Receptor antibody (A5865) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)}

Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunohistochemistry of paraffin-embedded human colon carcinoma using CD71/Transferrin Receptor antibody (A5865) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)}

Immunohistochemistry - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunohistochemistry of paraffin-embedded human placenta using CD71/Transferrin Receptor antibody (A5865) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - CD71/Transferrin Receptor Rabbit pAb (A5865)}

Immunofluorescence - CD71/Transferrin Receptor Rabbit pAb (A5865)

Immunofluorescence analysis of PC-12 cells using CD71/Transferrin Receptor Rabbit pAb (A5865) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on TFRC. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TFRC. (Distance between topics and target gene indicate popularity.) TFRC

* Data provided by citexs.com, for reference only.

Publishing research using A5865? Please let us know so that we can cite the reference in this datasheet.

Proteins (1)

ELISA Kits (1)

Primary Antibodies (3)

Secondary Antibodies (26)

* For research use only. Not for therapeutic or diagnostic purposes.

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