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CD63 Rabbit pAb (A5271)

Reviews (2)Publications (16) Datasheet

ABclonal:CD63 Rabbit pAb
ABclonal:Western blot - CD63 Rabbit pAb (A5271)

Western blot analysis of extracts of various cell lines, using CD63 antibody (A5271) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - CD63 Rabbit pAb (A5271)

Immunohistochemistry of paraffin-embedded rat brain using CD63 Rabbit pAb (A5271) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - CD63 Rabbit pAb (A5271)

Immunofluorescence analysis of A431 cells using CD63 Rabbit pAb (A5271) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for CD63 Rabbit pAb(A5271)
Review
ABclonal: review for CD63 Rabbit pAb(A5271)

Overview

Product nameCD63 Rabbit pAb
Catalog No.A5271
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 103-203 of human CD63 (NP_001771.1).
SequenceAGYVFRDKVMSEFNNNFRQQMENYPKNNHTASILDRMQADFKCCGAANYTDWEKIPSMSKNRVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNV
Gene ID967
Swiss protP08962
SynonymsCD63; LAMP-3; ME491; MLA1; OMA81H; TSPAN30; CD63 antigen; CD63
Calculated MW17kDa/23kDa/25kDa
Observed MW30-60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHL-60, RAW264.7, Rat brain
Cellular locationCell membrane, Endosome, Late endosome membrane, Lysosome membrane, Melanosome, Multi-pass membrane protein, multivesicular body
Customer validation

(human and mouse cells, HEK293T)

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Cynoglossus semilaevis, Danio rerio)

IHC(Rattus norvegicus)

ABclonal:Western blot - CD63 Rabbit pAb (A5271) }

Western blot - CD63 Rabbit pAb (A5271)

Western blot analysis of extracts of various cell lines, using CD63 antibody (A5271) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - CD63 Rabbit pAb (A5271) }

Immunohistochemistry - CD63 Rabbit pAb (A5271)

Immunohistochemistry of paraffin-embedded rat brain using CD63 Rabbit pAb (A5271) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - CD63 Rabbit pAb (A5271) }

Immunofluorescence - CD63 Rabbit pAb (A5271)

Immunofluorescence analysis of A431 cells using CD63 Rabbit pAb (A5271) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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