Publications (12) Datasheet COA
Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Reactivity:Human, Mouse, Rat
Western blot analysis of lysates from U-937 cells, using CD4 Rabbit pAb (A0362) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of Mouse thymus using CD4 Rabbit pAb (A0362) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Rat thymus cells using CD4 Rabbit pAb (A0362) at dilution of 1:100. Blue: DAPI for nuclear staining.
| Product name | CD4 Rabbit pAb |
|---|---|
| Catalog No. | A0362 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 26-200 of human CD4 (NP_000607.1). |
|---|---|
| Sequence | KKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIV |
| Gene ID | 920 |
| Swiss prot | P01730 |
| Synonyms | T4; IMD79; Leu-3; OKT4D; CD4mut; CD4 |
| Calculated MW | 51kDa |
| Observed MW | 51kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunofluorescence |
| Positive samples | U-937 |
| Cellular location | Cell membrane, Single-pass type I membrane protein |
| Customer validation | IHC (Mus musculus, Homo sapiens) IF (Mus musculus) WB (Mus musculus) IF (Mus musculus) |
To download a Certificate of Compliance, please enter your Lot number below:
Lot number
Submit your question about A0362 below and we will get back to you within one business day.
Alternatively, call us at 888.754.5670.
Based on existing publications, the following genes are closely related to research on CD4. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to CD4. (Distance between topics and target gene indicate popularity.) CD4
* Data provided by citexs.com, for reference only.
Publishing research using A0362? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.
i_FC_01.jpg?t=1698043657)
_WB_01.jpg?t=1698886134)
_WB_01.jpg?t=1701998521)
