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CD4 Polyclonal Antibody (A1549)

Datasheet

ABclonal:Western blot - CD4 Polyclonal Antibody (A1549)

Overview

Product nameCD4 Polyclonal Antibody
Catalog No.A1549
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300-400 of human CD4 (NP_000607.1).
SequenceALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLMLSLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMALI
Gene ID920
Swiss protP01730
SynonymsCD4; CD4mut; CD4 molecule
Calculated MW51kDa
Observed MW55kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesSW620, Mouse brain
Cellular locationCell membrane, Single-pass type I membrane protein
ABclonal:Western blot - CD4 Polyclonal Antibody (A1549) }

Western blot - CD4 Polyclonal Antibody (A1549)

Western blot analysis of extracts of various cell lines, using CD4 antibody (A1549) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

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