Review (1) Publications (11) Datasheet COA
Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Reactivity:Human, Mouse
Western blot analysis of extracts of various cell lines, using CD44 antibody (A1351) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded human tonsil using CD44 antibody (A1351) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using CD44 antibody (A1351) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
| Product name | CD44 Rabbit pAb |
|---|---|
| Catalog No. | A1351 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 20-178 of CD44 (NP_000601.3). |
|---|---|
| Sequence | AQIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDV |
| Gene ID | 960 |
| Swiss prot | P16070 |
| Synonyms | IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; H-CAM; HCELL; ECM-III; HUTCH-1; HUTCH-I; ECMR-III; Hermes-1; CD44 |
| Calculated MW | 82kDa |
| Observed MW | 82kDa |
| Reactivity | Human, Mouse |
|---|---|
| Tested applications | WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry |
| Positive samples | MCF7, HeLa, Raji, A-549, U-251MG, Mouse liver, Mouse spleen |
| Cellular location | Cell membrane, Single-pass type I membrane protein |
| Customer validation | WB (unknow, Homo sapiens, Mus musculus, Gallus gallus) IF (Homo sapiens) IHC (Homo sapiens) |
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Based on existing publications, the following genes are closely related to research on CD44. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to CD44. (Distance between topics and target gene indicate popularity.) CD44
* Data provided by citexs.com, for reference only.
Publishing research using A1351? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.
![[KO Validated] CD44 Rabbit pAb](https://abclonal-us.oss-us-east-1.aliyuncs.com/abclonal/Catalog/A12410/A12410_1.jpg?t=1690972960)
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