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CD44 Rabbit pAb (A1351)

Review (1)Publications (6) Datasheet

ABclonal:Western blot - CD44 Polyclonal Antibody (A1351)

Western blot analysis of extracts of various cell lines, using CD44 antibody (A1351) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - CD44 Polyclonal Antibody (A1351)

Immunohistochemistry of paraffin-embedded human tonsil using CD44 antibody (A1351) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - CD44 Polyclonal Antibody (A1351)

Immunohistochemistry of paraffin-embedded human colon carcinoma using CD44 antibody (A1351) at dilution of 1:200 (40x lens).

Review
ABclonal: review for CD44 Rabbit pAb(A1351)

Overview

Product nameCD44 Rabbit pAb
Catalog No.A1351
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 35-300 of human CD44 (NP_001001389.1).
SequenceHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIPATSTSSNTISAGWEPNEENEDERDRHLSFSGSGIDDDEDFISSTISTTPRAFDHTKQNQDWTQWNPSHSNPEVLLQTTTR
Gene ID960
Swiss protP16070
SynonymsCDW44; CSPG8; ECMR-III; HCELL; HUTCH-I; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CD44; CD44
Calculated MW3kDa/15kDa/32-53kDa/73-81kDa
Observed MW82kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesMCF7, HeLa, Raji, A-549, U-251MG, Mouse liver, Mouse spleen
Cellular locationCell membrane, Single-pass type I membrane protein
Customer validation

(unknow)

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - CD44 Polyclonal Antibody (A1351) }

Western blot - CD44 Polyclonal Antibody (A1351)

Western blot analysis of extracts of various cell lines, using CD44 antibody (A1351) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - CD44 Polyclonal Antibody (A1351) }

Immunohistochemistry - CD44 Polyclonal Antibody (A1351)

Immunohistochemistry of paraffin-embedded human tonsil using CD44 antibody (A1351) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - CD44 Polyclonal Antibody (A1351) }

Immunohistochemistry - CD44 Polyclonal Antibody (A1351)

Immunohistochemistry of paraffin-embedded human colon carcinoma using CD44 antibody (A1351) at dilution of 1:200 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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