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CD44 Rabbit pAb (A0340)

Datasheet

ABclonal:Western blot - CD44 Rabbit pAb (A0340)
ABclonal:Immunohistochemistry - CD44 Rabbit pAb (A0340)
ABclonal:Immunohistochemistry - CD44 Rabbit pAb (A0340)
ABclonal:Immunohistochemistry - CD44 Rabbit pAb (A0340)
ABclonal:Immunoprecipitation - CD44 Rabbit pAb (A0340)

Overview

Product nameCD44 Rabbit pAb
Catalog No.A0340
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 346-385 of human CD44 (NP_000601.3).
SequenceDVDRNGTTAYEGNWNPEAHPPLIHHEHHEEEETPHSTSTI
Gene ID960
Swiss protP16070
SynonymsCDW44; CSPG8; ECMR-III; HCELL; HUTCH-I; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CD44; CD44
Calculated MW3kDa/15kDa/32-53kDa/73-81kDa
Observed MW82kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000IHC 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesK-562, HeLa, HT-1080
Cellular locationCell membrane, Single-pass type I membrane protein
ABclonal:Western blot - CD44 Rabbit pAb (A0340) }

Western blot - CD44 Rabbit pAb (A0340)

Western blot analysis of extracts of various cell lines, using CD44 antibody (A0340) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - CD44 Rabbit pAb (A0340) }

Immunohistochemistry - CD44 Rabbit pAb (A0340)

Immunohistochemistry of paraffin-embedded human tonsil using CD44 antibody (A0340) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - CD44 Rabbit pAb (A0340) }

Immunohistochemistry - CD44 Rabbit pAb (A0340)

Immunohistochemistry of paraffin-embedded human colon carcinoma using CD44 antibody (A0340) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - CD44 Rabbit pAb (A0340) }

Immunohistochemistry - CD44 Rabbit pAb (A0340)

Immunohistochemistry of paraffin-embedded human gastric cancer using CD44 antibody (A0340) at dilution of 1:200 (40x lens).
ABclonal:Immunoprecipitation - CD44 Rabbit pAb (A0340) }

Immunoprecipitation - CD44 Rabbit pAb (A0340)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug CD44 antibody (A0340). Western blot was performed from the immunoprecipitate using CD44 antibody (A0340) at a dilition of 1:1000.

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