Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CD36/SR-B3 Rabbit pAb (A5792)

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - CD36/SR-B3 Rabbit pAb (A5792)

Western blot analysis of various lysates using CD36/SR-B3 Rabbit pAb (A5792) at 1:2000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 60s.

ABclonal:Immunohistochemistry - CD36/SR-B3 Rabbit pAb (A5792)

Immunohistochemistry analysis of CD36/SR-B3 in paraffin-embedded human liver tissue using CD36/SR-B3 Rabbit pAb (A5792) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of CD36/SR-B3 in paraffin-embedded human spleen tissue using CD36/SR-B3 Rabbit pAb (A5792) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

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Overview

Product name	CD36/SR-B3 Rabbit pAb
Catalog No.	A5792
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 30-439 of human CD36/SR-B3 (NP_000063.2).
Sequence	GDLLIQKTIKKQVVLEEGTIAFKNWVKTGTEVYRQFWIFDVQNPQEVMMNSSNIQVKQRGPYTYRVRFLAKENVTQDAEDNTVSFLQPNGAIFEPSLSVGTEADNFTVLNLAVAAASHIYQNQFVQMILNSLINKSKSSMFQVRTLRELLWGYRDPFLSLVPYPVTTTVGLFYPYNNTADGVYKVFNGKDNISKVAIIDTYKGKRNLSYWESHCDMINGTDAASFPPFVEKSQVLQFFSSDICRSIYAVFESDVNLKGIPVYRFVLPSKAFASPVENPDNYCFCTEKIISKNCTSYGVLDISKCKEGRPVYISLPHFLYASPDVSEPIDGLNPNEEEHRTYLDIEPITGFTLQFAKRLQVNLLVKPSEKIQVLKNLKRNYIVPILWLNETGTIGDEKANMFRSQVTGKIN
Gene ID	948
Swiss prot	P16671
Synonyms	FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10; CD36/SR-B3
Calculated MW	53kDa
Observed MW	70-110kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry
Positive samples	Mouse heart, Rat heart
Cellular location	Cell membrane, Multi-pass membrane protein
Customer validation	WB (Mus musculus, Rattus norvegicus, Gallus gallus) IF (Mus musculus) IF (Mus musculus) sCD36 level was measured (Homo sapiens)

Research Area

CD36/SR-B3 Rabbit pAb images

Western blot - CD36/SR-B3 Rabbit pAb (A5792)

Immunohistochemistry - CD36/SR-B3 Rabbit pAb (A5792)

Product Protocols

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Related Targets

Based on existing publications, the following genes are closely related to research on CD36. (Numbers indicate number of published articles where both targets appear.)

◀1/▶

Related Research Area

Based on existing publications, the following research topics are related to CD36. (Distance between topics and target gene indicate popularity.) CD36

* Data provided by citexs.com, for reference only.

References for CD36/SR-B3 Rabbit pAb (A5792)

Product:

CD36/SR-B3 Rabbit pAb

Journal:

Cardiovascular diabetology

Application:

IF:

9.951

Species:

Mus musculus

PMID:

30635067

Title:

MiR-30c/PGC-1β protects against diabetic cardiomyopathy via PPARα.

References for CD36/SR-B3 Rabbit pAb (A5792)

Product:

CD36/SR-B3 Rabbit pAb

Journal:

Mediators of inflammation

Application:

IF:

4.711

Species:

Mus musculus

PMID:

31379468

Title:

Shexiang Baoxin Pill Alleviates the Atherosclerotic Lesions in Mice via Improving Inflammation Response and Inhibiting Lipid Accumulation in the Arterial Wall.

References for CD36/SR-B3 Rabbit pAb (A5792)

Product:

CD36/SR-B3 Rabbit pAb

Journal:

Theranostics

Application:

IF:

11.556

Species:

Mus musculus

PMID:

32373236

Title:

Branched chain amino acids exacerbate myocardial ischemia/reperfusion vulnerability via enhancing GCN2/ATF6/PPAR-α pathway-dependent fatty acid oxidation

References for CD36/SR-B3 Rabbit pAb (A5792)

Product:

CD36/SR-B3 Rabbit pAb

Journal:

Biomed Pharmacother

Application:

IF:

6.529

Species:

Mus musculus

PMID:

32554245

Title:

1, 2, 3, 4, 6 penta-O-galloyl-β-D-glucose ameliorates high-fat diet-induced nonalcoholic fatty liver disease and maintains the expression of genes involved in lipid homeostasis in mice

References for CD36/SR-B3 Rabbit pAb (A5792)

Product:

CD36/SR-B3 Rabbit pAb

Journal:

Aging-US

Application:

IF:

5.682

Species:

Mus musculus

PMID:

33186123

Title:

The nuclear and cytoplasmic roles of miR-320 in non-alcoholic fatty liver disease.

References for CD36/SR-B3 Rabbit pAb (A5792)

Product:

CD36/SR-B3 Rabbit pAb

Journal:

Front Cell Dev Biol

Application:

IF:

6.684

Species:

Rattus norvegicus

PMID:

34881240

Title:

LuHui Derivative, A Novel Compound That Inhibits the Fat Mass and Obesity-Associated (FTO), Alleviates the Inflammatory Response and Injury in Hyperlipidemia-Induced Cardiomyopathy

References for CD36/SR-B3 Rabbit pAb (A5792)

Product:

CD36/SR-B3 Rabbit pAb

Journal:

Front Physiol

Application:

IF:

4.566

Species:

Gallus gallus

PMID:

34744775

Title:

Effects of Cyclic Chronic Heat Stress on the Expression of Nutrient Transporters in the Jejunum of Modern Broilers and Their Ancestor Wild Jungle Fowl

References for CD36/SR-B3 Rabbit pAb (A5792)

Product:

CD36/SR-B3 Rabbit pAb

Journal:

Cell & bioscience

Application:

WB, IF

IF:

9.58

Species:

Mus musculus

PMID:

35619150

Title:

β-amyloid protein induces mitophagy-dependent ferroptosis through the CD36/PINK/PARKIN pathway leading to blood-brain barrier destruction in Alzheimer's disease

References for CD36/SR-B3 Rabbit pAb (A5792)

Product:

CD36/SR-B3 Rabbit pAb

Journal:

Molecular biotechnology

Application:

IF:

2.86

Species:

Mus musculus

PMID:

35435591

Title:

MiR-199a-3p Restrains Foaming and Inflammation by Regulating RUNX1 in Macrophages

References for CD36/SR-B3 Rabbit pAb (A5792)

Product:

CD36/SR-B3 Rabbit pAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.65

Species:

Mus musculus

PMID:

36682299

Title:

Diosgenin attenuates non-alcoholic fatty liver disease in type 2 diabetes through regulating SIRT6-related fatty acid uptake

References for CD36/SR-B3 Rabbit pAb (A5792)

Product:

CD36/SR-B3 Rabbit pAb

Journal:

Journal of molecular and cellular cardiology

Application:

IF:

5.76

Species:

Mus musculus

PMID:

36509022

Title:

Klf4 deficiency exacerbates myocardial ischemia/reperfusion injury in mice via enhancing ROCK1/DRP1 pathway-dependent mitochondrial fission

References for CD36/SR-B3 Rabbit pAb (A5792)

Product:

CD36/SR-B3 Rabbit pAb

Journal:

Food & function

Application:

IF:

6.31

Species:

Mus musculus

PMID:

36786689

Title:

(-)-Epicatechin gallate blocked cellular foam formation in atherosclerosis by modulating CD36 expression in vitro and in vivo

References for CD36/SR-B3 Rabbit pAb (A5792)

Product:

CD36/SR-B3 Rabbit pAb

Journal:

Cardiovascular revascularization medicine : including molecular interventions

Application:

sCD36 level was measured

IF:

Species:

Homo sapiens

PMID:

37142533

Title:

The addition of evolocumab to maximal tolerated statin therapy improves walking performance in patients with peripheral arterial disease and intermittent claudication …

References for CD36/SR-B3 Rabbit pAb (A5792)

Product:

CD36/SR-B3 Rabbit pAb

Journal:

NPJ Regenerative medicine

Application:

IF:

14.4

Species:

Mus musculus

PMID:

37291182