Western blot analysis of extracts of various cell lines, using CD36/SR-B3 antibody (A17339) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human liver using CD36/SR-B3 Rabbit pAb (A17339) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using CD36/SR-B3 Rabbit pAb (A17339) at dilution of 1:100 (40x lens).
|Product name||CD36/SR-B3 Rabbit pAb|
|Purification method||Affinity purification|
|Reactivity||Human, Mouse, Rat|
|Tested applications||WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP|
|Recommended dilution||WB 1:500 - 1:2000|
IHC 1:10 - 1:100
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.|
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting Immunohistochemistry|
|Positive samples||THP-1, RAW264.7, Rat fat|
|Cellular location||Cell membrane, Multi-pass membrane protein|
* For research use only. Not for therapeutic or diagnostic purposes.
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