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CD36/SR-B3 Rabbit pAb (A17339)

Publication (1) Datasheet

ABclonal:Western blot - CD36/SR-B3 Rabbit pAb (A17339)

Western blot analysis of extracts of various cell lines, using CD36/SR-B3 antibody (A17339) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - CD36/SR-B3 Rabbit pAb (A17339)

Immunohistochemistry of paraffin-embedded human liver using CD36/SR-B3 Rabbit pAb (A17339) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CD36/SR-B3 Rabbit pAb (A17339)

Immunohistochemistry of paraffin-embedded mouse liver using CD36/SR-B3 Rabbit pAb (A17339) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CD36/SR-B3 Rabbit pAb (A17339)

Immunohistochemistry of paraffin-embedded rat spleen using CD36/SR-B3 Rabbit pAb (A17339) at dilution of 1:100 (40x lens).

Overview

Product nameCD36/SR-B3 Rabbit pAb
Catalog No.A17339
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene.
ImmunogenRecombinant protein of human CD36/SR-B3.
SequenceEmail for sequence
Gene ID948
Swiss protP16671
SynonymsBDPLT10; CHDS7; FAT; GP3B; GP4; GPIV; PASIV; SCARB3; CD36
Calculated MW32kDa/46kDa/48kDa/53kDa
Observed MW70-110KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesTHP-1, RAW264.7, Rat fat
Cellular locationCell membrane, Multi-pass membrane protein
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - CD36/SR-B3 Rabbit pAb (A17339) }

Western blot - CD36/SR-B3 Rabbit pAb (A17339)

Western blot analysis of extracts of various cell lines, using CD36/SR-B3 antibody (A17339) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - CD36/SR-B3 Rabbit pAb (A17339) }

Immunohistochemistry - CD36/SR-B3 Rabbit pAb (A17339)

Immunohistochemistry of paraffin-embedded human liver using CD36/SR-B3 Rabbit pAb (A17339) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CD36/SR-B3 Rabbit pAb (A17339) }

Immunohistochemistry - CD36/SR-B3 Rabbit pAb (A17339)

Immunohistochemistry of paraffin-embedded mouse liver using CD36/SR-B3 Rabbit pAb (A17339) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CD36/SR-B3 Rabbit pAb (A17339) }

Immunohistochemistry - CD36/SR-B3 Rabbit pAb (A17339)

Immunohistochemistry of paraffin-embedded rat spleen using CD36/SR-B3 Rabbit pAb (A17339) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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