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CD36 Rabbit pAb (A1470)

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ABclonal:Western blot - CD36 Rabbit pAb (A1470)
ABclonal: review for CD36 Rabbit pAb(A1470)


Product nameCD36 Rabbit pAb
Catalog No.A1470
Host speciesRabbit
Purification methodAffinity purification
The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 301-400 of human CD36 (NP_001120916.1).
Gene ID948
Swiss protP16671
Calculated MW32kDa/46kDa/48kDa/53kDa
Observed MW70-110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T, Mouse heart, Rat heart
Cellular locationCell membrane, Multi-pass membrane protein
Customer validation

(rat liver)

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - CD36 Rabbit pAb (A1470) }

Western blot - CD36 Rabbit pAb (A1470)

Western blot analysis of extracts of various cell lines, using CD36 antibody (A1470) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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