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CD34 Rabbit pAb (A13929)

Publications (2) Datasheet

ABclonal:Western blot - CD34 Rabbit pAb (A13929)

Western blot analysis of extracts of various cell lines, using CD34 antibody (A13929) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - CD34 Rabbit pAb (A13929)

Immunohistochemistry of paraffin-embedded human placenta using CD34 antibody (A13929) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CD34 Rabbit pAb (A13929)

Immunohistochemistry of paraffin-embedded mouse kidney using CD34 antibody (A13929) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - CD34 Rabbit pAb (A13929)

Immunohistochemistry of paraffin-embedded rat kidney using CD34 antibody (A13929) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - CD34 Rabbit pAb (A13929)

Immunofluorescence analysis of rat brain using CD34 antibody (A13929) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CD34 Rabbit pAb (A13929)

Immunofluorescence analysis of human placenta using CD34 antibody (A13929) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameCD34 Rabbit pAb
Catalog No.A13929
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human CD34 (NP_001020280.1).
SequenceLLAVLGITGYFLMNRRSWSPTGERLGEDPYYTENGGGQGYSSGPGTSPEAQGKASVNRGAQENGTGQATSRNGHSARQHVVADTEL
Gene ID947
Swiss protP28906
SynonymsCD34; CD34 molecule; GIG3; MORT1
Calculated MW35kDa/40kDa
Observed MW105KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse thymus, Rat lung, Rat heart
Cellular locationMembrane, Single-pass type I membrane protein
Customer validation

IHC(Homo sapiens,Mus musculus)

ABclonal:Western blot - CD34 Rabbit pAb (A13929) }

Western blot - CD34 Rabbit pAb (A13929)

Western blot analysis of extracts of various cell lines, using CD34 antibody (A13929) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - CD34 Rabbit pAb (A13929) }

Immunohistochemistry - CD34 Rabbit pAb (A13929)

Immunohistochemistry of paraffin-embedded human placenta using CD34 antibody (A13929) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CD34 Rabbit pAb (A13929) }

Immunohistochemistry - CD34 Rabbit pAb (A13929)

Immunohistochemistry of paraffin-embedded mouse kidney using CD34 antibody (A13929) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CD34 Rabbit pAb (A13929) }

Immunohistochemistry - CD34 Rabbit pAb (A13929)

Immunohistochemistry of paraffin-embedded rat kidney using CD34 antibody (A13929) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - CD34 Rabbit pAb (A13929) }

Immunofluorescence - CD34 Rabbit pAb (A13929)

Immunofluorescence analysis of rat brain using CD34 antibody (A13929) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CD34 Rabbit pAb (A13929) }

Immunofluorescence - CD34 Rabbit pAb (A13929)

Immunofluorescence analysis of human placenta using CD34 antibody (A13929) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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