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CD34 Polyclonal Antibody (A0761)

Review (1)Publications (3) Datasheet

ABclonal:Western blot - CD34 Polyclonal Antibody (A0761)
ABclonal:Immunohistochemistry - CD34 Polyclonal Antibody (A0761)
ABclonal:Immunofluorescence - CD34 Polyclonal Antibody (A0761)
Review
ABclonal: review for CD34 Polyclonal Antibody(A0761)

Overview

Product nameCD34 Polyclonal Antibody
Catalog No.A0761
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 32-290 of human CD34 (NP_001020280.1).
SequenceSLDNNGTATPELPTQGTFSNVSTNVSYQETTTPSTLGSTSLHPVSQHGNEATTNITETTVKFTSTSVITSVYGNTNSSVQSQTSVISTVFTTPANVSTPETTLKPSLSPGNVSDLSTTSTSLATSPTKPYTSSSPILSDIKAEIKCSGIREVKLTQGICLEQNKTSSCAEFKKDRGEGLARVLCGEEQADADAGAQVCSLLLAQSEVRPQCLLLVLANRTEISSKLQLMKKHQSDLKKLGILDFTEQDVASHQSYSQKT
Gene ID947
Swiss protP28906
SynonymsCD34; CD34 molecule
Calculated MW35kDa/40kDa
Observed MW115-125kDa
ReactivityHuman, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-431, Rat testis
Cellular locationMembrane, Single-pass type I membrane protein
Customer validation

WB(Human, Mouse)

IHC(Human)

ABclonal:Western blot - CD34 Polyclonal Antibody (A0761) }

Western blot - CD34 Polyclonal Antibody (A0761)

Western blot analysis of extracts of various cell lines, using CD34 Antibody (A0761) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - CD34 Polyclonal Antibody (A0761) }

Immunohistochemistry - CD34 Polyclonal Antibody (A0761)

Immunohistochemistry of paraffin-embedded human appendix using CD34 antibody (A0761) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - CD34 Polyclonal Antibody (A0761) }

Immunofluorescence - CD34 Polyclonal Antibody (A0761)

Immunofluorescence analysis of human placenta using CD34 antibody (A0761) at dilution of 1:100. Blue: DAPI for nuclear staining.

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