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CD31 Rabbit pAb (A2104)

Publication (2) Datasheet

ABclonal:Western blot - CD31 Polyclonal Antibody (A2104)
ABclonal:Immunohistochemistry - CD31 Polyclonal Antibody (A2104)
ABclonal:Immunohistochemistry - CD31 Polyclonal Antibody (A2104)
ABclonal:Immunohistochemistry - CD31 Polyclonal Antibody (A2104)

Overview

Product nameCD31 Rabbit pAb
Catalog No.A2104
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 130-230 of human CD31 (NP_000433.4).
SequenceVPSPRVTLDKKEAIQGGIVRVNCSVPEEKAPIHFTIEKLELNEKMVKLKREKNSRDQNFVILEFPVEEQDRVLSFRCQARIISGIHMQTSESTKSELVTVT
Gene ID5175
Swiss protP16284
SynonymsCD31; CD31/EndoCAM; PECA1; PECAM-1; endoCAM; PECAM1
Calculated MW79kDa/80kDa/81kDa/82kDa
Observed MW130kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesTHP-1
Cellular locationCell junction, Cell junction, Cell membrane, Lipid-anchor, Single-pass type I membrane protein
Customer validation

IHC(Human, Mouse)

ABclonal:Western blot - CD31 Polyclonal Antibody (A2104) }

Western blot - CD31 Polyclonal Antibody (A2104)

Western blot analysis of extracts of THP-1 cells, using CD31 antibody (A2104) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - CD31 Polyclonal Antibody (A2104) }

Immunohistochemistry - CD31 Polyclonal Antibody (A2104)

Immunohistochemistry of paraffin-embedded human liver cancer using CD31 antibody (A2104) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CD31 Polyclonal Antibody (A2104) }

Immunohistochemistry - CD31 Polyclonal Antibody (A2104)

Immunohistochemistry of paraffin-embedded human liver damage using CD31 antibody (A2104) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - CD31 Polyclonal Antibody (A2104) }

Immunohistochemistry - CD31 Polyclonal Antibody (A2104)

Immunohistochemistry of paraffin-embedded human kidney damage using CD31 antibody (A2104) at dilution of 1:100 (40x lens).

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