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CD31/PECAM1 Rabbit pAb (A2104)

Publications (3) Datasheet

ABclonal:Western blot - CD31/PECAM1 Rabbit pAb (A2104)

Western blot analysis of extracts of Jurkat cells, using CD31/PECAM1 antibody (A2104) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - CD31/PECAM1 Rabbit pAb (A2104)

Immunohistochemistry of paraffin-embedded Human placenta using CD31/PECAM1 Rabbit pAb (A2104) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - CD31/PECAM1 Rabbit pAb (A2104)

Immunofluorescence analysis of human placenta using CD31/PECAM1 Rabbit pAb (A2104) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameCD31/PECAM1 Rabbit pAb
Catalog No.A2104
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 130-230 of human CD31/PECAM1 (NP_000433.4).
SequenceVPSPRVTLDKKEAIQGGIVRVNCSVPEEKAPIHFTIEKLELNEKMVKLKREKNSRDQNFVILEFPVEEQDRVLSFRCQARIISGIHMQTSESTKSELVTVT
Gene ID5175
Swiss protP16284
SynonymsCD31; CD31/EndoCAM; PECA1; PECAM-1; endoCAM; PECAM1
Calculated MW79kDa/80kDa/81kDa/82kDa
Observed MW130KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat
Cellular locationCell junction, Cell junction, Cell membrane, Lipid-anchor, Single-pass type I membrane protein
Customer validation

IF(Rattus norvegicus)

IHC(Homo sapiens, Mus musculus)

ABclonal:Western blot - CD31/PECAM1 Rabbit pAb (A2104) }

Western blot - CD31/PECAM1 Rabbit pAb (A2104)

Western blot analysis of extracts of Jurkat cells, using CD31/PECAM1 antibody (A2104) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - CD31/PECAM1 Rabbit pAb (A2104) }

Immunohistochemistry - CD31/PECAM1 Rabbit pAb (A2104)

Immunohistochemistry of paraffin-embedded Human placenta using CD31/PECAM1 Rabbit pAb (A2104) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - CD31/PECAM1 Rabbit pAb (A2104) }

Immunofluorescence - CD31/PECAM1 Rabbit pAb (A2104)

Immunofluorescence analysis of human placenta using CD31/PECAM1 Rabbit pAb (A2104) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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