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CD26/DPP4 Rabbit mAb (A4252)

Publication (1) Datasheet

ABclonal:Western blot - CD26/DPP4 Rabbit mAb (A4252)

Western blot analysis of extracts of various cell lines, using CD26/DPP4 Rabbit mAb (A4252) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

Overview

Product nameCD26/DPP4 Rabbit mAb
Catalog No.A4252
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. [provided by RefSeq, Jul 2008]
ImmunogenA synthesized peptide derived from human CD26/DPP4
SequenceEmail for sequence
Gene ID1803
Swiss protP27487
SynonymsADABP; ADCP2; CD26; DPPIV; TP103
Calculated MW110kDa
Observed MW105KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesU-937, Mouse liver, Rat lung, Rat liver, Rat kidney
Cellular locationApical cell membrane, Cell junction, Cell membrane, Cell projection, Membrane raft, Secreted, Single-pass type II membrane protein, invadopodium membrane, lamellipodium membrane
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - CD26/DPP4 Rabbit mAb (A4252) }

Western blot - CD26/DPP4 Rabbit mAb (A4252)

Western blot analysis of extracts of various cell lines, using CD26/DPP4 Rabbit mAb (A4252) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

* For research use only. Not for therapeutic or diagnostic purposes.

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