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CD20 Rabbit pAb (A1793)

Publications (3) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse

ABclonal:Western blot - CD20 Rabbit pAb (A1793)

Western blot analysis of extracts of Raji cells, using CD20 antibody (A1793) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - CD20 Rabbit pAb (A1793)

Immunohistochemistry of paraffin-embedded human tonsil using CD20 Rabbit pAb (A1793) at dilution of 1:150 (40x lens).

ABclonal:Immunofluorescence - CD20 Rabbit pAb (A1793)

Immunofluorescence analysis of Raji cells using CD20 Rabbit pAb (A1793) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameCD20 Rabbit pAb
Catalog No.A1793
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 208-297 of human CD20 (NP_068769.2).
SequenceIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP
Gene ID931
Swiss protP11836
SynonymsMS4A1; B1; Bp35; CD20; CVID5; LEU-16; MS4A2; S7
Calculated MW14kDa/33kDa
Observed MW33-37kd
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRaji
Cellular locationCell membrane, Lipid-anchor, Multi-pass membrane protein
Customer validation

IHC(Mus musculus, Homo sapiens)

IF(Mus musculus)

ABclonal:Western blot - CD20 Rabbit pAb (A1793) }

Western blot - CD20 Rabbit pAb (A1793)

Western blot analysis of extracts of Raji cells, using CD20 antibody (A1793) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - CD20 Rabbit pAb (A1793) }

Immunohistochemistry - CD20 Rabbit pAb (A1793)

Immunohistochemistry of paraffin-embedded human tonsil using CD20 Rabbit pAb (A1793) at dilution of 1:150 (40x lens).
ABclonal:Immunofluorescence - CD20 Rabbit pAb (A1793) }

Immunofluorescence - CD20 Rabbit pAb (A1793)

Immunofluorescence analysis of Raji cells using CD20 Rabbit pAb (A1793) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

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