Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

CD141/Thrombomodulin Rabbit mAb (A21197)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - CD141/Thrombomodulin Rabbit mAb (A21197)

Western blot analysis of extracts of A-431 cells, using CD141/Thrombomodulin antibody (A21197) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunofluorescence - CD141/Thrombomodulin Rabbit mAb (A21197)

Immunofluorescence analysis of A431 using CD141/Thrombomodulin Rabbit mAb (A21197) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CD141/Thrombomodulin Rabbit mAb (A21197)

Immunofluorescence analysis of THP-1 using CD141/Thrombomodulin Rabbit mAb (A21197) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Flow CytoMetry - CD141/Thrombomodulin Rabbit mAb (A21197)

Flow cytometry:1X10^6 Jurkat cells (negative control, left) and THP-1 cells (right) were surface-stained with CD141/Thrombomodulin Rabbit mAb(A21197, 10 μg/mL, green line) or Rabbit IgG isotype control (AC042, 10 μg/mL, blue line), followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).

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Overview

Product name CD141/Thrombomodulin Rabbit mAb
Catalog No. A21197
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC52599

Background

The protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 19-515 of human CD141/Thrombomodulin (NP_000352.1).
Sequence APAEPQPGGSQCVEHDCFALYPGPATFLNASQICDGLRGHLMTVRSSVAADVISLLLNGDGGVGRRRLWIGLQLPPGCGDPKRLGPLRGFQWVTGDNNTSYSRWARLDLNGAPLCGPLCVAVSAAEATVPSEPIWEEQQCEVKADGFLCEFHFPATCRPLAVEPGAAAAAVSITYGTPFAARGADFQALPVGSSAAVAPLGLQLMCTAPPGAVQGHWAREAPGAWDCSVENGGCEHACNAIPGAPRCQCPAGAALQADGRSCTASATQSCNDLCEHFCVPNPDQPGSYSCMCETGYRLAADQHRCEDVDDCILEPSPCPQRCVNTQGGFECHCYPNYDLVDGECVEPVDPCFRANCEYQCQPLNQTSYLCVCAEGFAPIPHEPHRCQMFCNQTACPADCDPNTQASCECPEGYILDDGFICTDIDECENGGFCSGVCHNLPGTFECICGPDSALARHIGTDCDSGKVDGGDSGSGEPPPSPTPGSTLTPPAVGLVHS
Gene ID 7056
Swiss prot P07204
Synonyms TM; THRM; AHUS6; BDCA3; CD141; BDCA-3; THPH12; CD141/Thrombomodulin
Calculated MW 60kDa
Observed MW 90kDa

Applications

Reactivity Human
Tested applications Testing results
IF/ICC Human
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
  • FC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    Flow Cytometry    
Positive samples A-431
Cellular location Endoplasmic reticulum membrane, Golgi apparatus membrane, Single-pass membrane protein

Documents

Certificate of Compliance

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Research Area

CD141/Thrombomodulin Rabbit mAb images

ABclonal:Western blot - CD141/Thrombomodulin Rabbit mAb (A21197)}

Western blot - CD141/Thrombomodulin Rabbit mAb (A21197)

Western blot analysis of extracts of A-431 cells, using CD141/Thrombomodulin antibody (A21197) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunofluorescence - CD141/Thrombomodulin Rabbit mAb (A21197)}

Immunofluorescence - CD141/Thrombomodulin Rabbit mAb (A21197)

Immunofluorescence analysis of A431 using CD141/Thrombomodulin Rabbit mAb (A21197) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CD141/Thrombomodulin Rabbit mAb (A21197)}

Immunofluorescence - CD141/Thrombomodulin Rabbit mAb (A21197)

Immunofluorescence analysis of THP-1 using CD141/Thrombomodulin Rabbit mAb (A21197) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Flow CytoMetry - CD141/Thrombomodulin Rabbit mAb (A21197)}

Flow CytoMetry - CD141/Thrombomodulin Rabbit mAb (A21197)

Flow cytometry:1X10^6 Jurkat cells (negative control, left) and THP-1 cells (right) were surface-stained with CD141/Thrombomodulin Rabbit mAb(A21197, 10 μg/mL, green line) or Rabbit IgG isotype control (AC042, 10 μg/mL, blue line), followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).

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Related Targets

Based on existing publications, the following genes are closely related to research on THBD. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to THBD. (Distance between topics and target gene indicate popularity.) THBD

* Data provided by citexs.com, for reference only.

Publishing research using A21197? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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