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CD13/ANPEP Rabbit pAb (A5662)

Publications (4) Datasheet

ABclonal:Western blot - CD13/ANPEP Rabbit pAb (A5662)

Western blot analysis of extracts of various cell lines, using CD13/CD13/ANPEP antibody (A5662) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - CD13/ANPEP Rabbit pAb (A5662)

Immunofluorescence analysis of NIH/3T3 cells using CD13/CD13/ANPEP Rabbit pAb (A5662) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CD13/ANPEP Rabbit pAb (A5662)

Immunofluorescence analysis of HeLa cells using CD13/CD13/ANPEP Rabbit pAb (A5662) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CD13/ANPEP Rabbit pAb (A5662)

Immunofluorescence analysis of C6 cells using CD13/CD13/ANPEP Rabbit pAb (A5662) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameCD13/ANPEP Rabbit pAb
Catalog No.A5662
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 668-967 of human CD13/CD13/ANPEP (NP_001141.2).
SequenceLASAHKVPVTLALNNTLFLIEERQYMPWEAALSSLSYFKLMFDRSEVYGPMKNYLKKQVTPLFIHFRNNTNNWREIPENLMDQYSEVNAISTACSNGVPECEEMVSGLFKQWMENPNNNPIHPNLRSTVYCNAIAQGGEEEWDFAWEQFRNATLVNEADKLRAALACSKELWILNRYLSYTLNPDLIRKQDATSTIISITNNVIGQGLVWDFVQSNWKKLFNDYGGGSFSFSNLIQAVTRRFSTEYELQQLEQFKKDNEETGFGSGTRALEQALEKTKANIKWVKENKEVVLQWFTENSK
Gene ID290
Swiss protP15144
SynonymsANPEP; APN; CD13; GP150; LAP1; P150; PEPN
Calculated MW109kDa
Observed MW180kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesTHP-1, Mouse kidney, Mouse small intestine
Cellular locationCell membrane, Cytoplasm, Single-pass type II membrane protein, cytosol
Customer validation

WB(Homo sapiens, Sus scrofa)

ABclonal:Western blot - CD13/ANPEP Rabbit pAb (A5662) }

Western blot - CD13/ANPEP Rabbit pAb (A5662)

Western blot analysis of extracts of various cell lines, using CD13/CD13/ANPEP antibody (A5662) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - CD13/ANPEP Rabbit pAb (A5662) }

Immunofluorescence - CD13/ANPEP Rabbit pAb (A5662)

Immunofluorescence analysis of NIH/3T3 cells using CD13/CD13/ANPEP Rabbit pAb (A5662) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CD13/ANPEP Rabbit pAb (A5662) }

Immunofluorescence - CD13/ANPEP Rabbit pAb (A5662)

Immunofluorescence analysis of HeLa cells using CD13/CD13/ANPEP Rabbit pAb (A5662) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CD13/ANPEP Rabbit pAb (A5662) }

Immunofluorescence - CD13/ANPEP Rabbit pAb (A5662)

Immunofluorescence analysis of C6 cells using CD13/CD13/ANPEP Rabbit pAb (A5662) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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