Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CD117/c-Kit Rabbit pAb (A0357)

Publications (6) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - CD117/c-Kit Rabbit pAb (A0357)

Western blot analysis of Mouse lung, using CD117/c-Kit antibody (A0357) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - CD117/c-Kit Rabbit pAb (A0357)

Immunohistochemistry analysis of paraffin-embedded human gastric cancer using CD117/c-Kit antibody (A0357) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CD117/c-Kit Rabbit pAb (A0357)

Immunohistochemistry analysis of paraffin-embedded mouse intestin using CD117/c-Kit antibody (A0357) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CD117/c-Kit Rabbit pAb (A0357)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD117/c-Kit antibody (A0357) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CD117/c-Kit Rabbit pAb (A0357)

Immunohistochemistry analysis of paraffin-embedded rat kidney using CD117/c-Kit antibody (A0357) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CD117/c-Kit Rabbit pAb (A0357)

Immunohistochemistry analysis of paraffin-embedded rat intestine using CD117/c-Kit antibody (A0357) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - CD117/c-Kit Rabbit pAb (A0357)

Immunofluorescence analysis of NIH/3T3 cells using CD117/c-Kit antibody (A0357) at dilution of 1:50. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CD117/c-Kit Rabbit pAb (A0357)

Immunofluorescence analysis of PC-12 cells using CD117/c-Kit antibody (A0357) at dilution of 1:50. Blue: DAPI for nuclear staining.

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Overview

Product name CD117/c-Kit Rabbit pAb
Catalog No. A0357
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 210-412 of human CD117/c-Kit (NP_000213.1).
Sequence AVPVVSVSKASYLLREGEEFTVTCTIKDVSSSVYSTWKRENSQTKLQEKYNSWHHGDFNYERQATLTISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMINTTVFVNDGENVDLIVEYEAFPKPEHQQWIYMNRTFTDKWEDYPKSENESNIRYVSELHLTRLKGTEGGTYTFLVSNSDVNAAIAFNVYVNTK
Gene ID 3815
Swiss prot P10721
Synonyms PBT; SCFR; C-Kit; CD117; MASTC; CD117/c-Kit
Calculated MW 110kDa
Observed MW 145kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples Mouse lung
Cellular location Cell membrane, Cytoplasm, Single-pass type I membrane protein
Customer validation

WB (Homo sapiens, Mus musculus)

IHC (Rattus norvegicus)

IF (Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

CD117/c-Kit Rabbit pAb images

ABclonal:Western blot - CD117/c-Kit Rabbit pAb (A0357)}

Western blot - CD117/c-Kit Rabbit pAb (A0357)

Western blot analysis of Mouse lung, using CD117/c-Kit antibody (A0357) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - CD117/c-Kit Rabbit pAb (A0357)}

Immunohistochemistry - CD117/c-Kit Rabbit pAb (A0357)

Immunohistochemistry analysis of paraffin-embedded human gastric cancer using CD117/c-Kit antibody (A0357) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CD117/c-Kit Rabbit pAb (A0357)}

Immunohistochemistry - CD117/c-Kit Rabbit pAb (A0357)

Immunohistochemistry analysis of paraffin-embedded mouse intestin using CD117/c-Kit antibody (A0357) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CD117/c-Kit Rabbit pAb (A0357)}

Immunohistochemistry - CD117/c-Kit Rabbit pAb (A0357)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD117/c-Kit antibody (A0357) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CD117/c-Kit Rabbit pAb (A0357)}

Immunohistochemistry - CD117/c-Kit Rabbit pAb (A0357)

Immunohistochemistry analysis of paraffin-embedded rat kidney using CD117/c-Kit antibody (A0357) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CD117/c-Kit Rabbit pAb (A0357)}

Immunohistochemistry - CD117/c-Kit Rabbit pAb (A0357)

Immunohistochemistry analysis of paraffin-embedded rat intestine using CD117/c-Kit antibody (A0357) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - CD117/c-Kit Rabbit pAb (A0357)}

Immunofluorescence - CD117/c-Kit Rabbit pAb (A0357)

Immunofluorescence analysis of NIH/3T3 cells using CD117/c-Kit antibody (A0357) at dilution of 1:50. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CD117/c-Kit Rabbit pAb (A0357)}

Immunofluorescence - CD117/c-Kit Rabbit pAb (A0357)

Immunofluorescence analysis of PC-12 cells using CD117/c-Kit antibody (A0357) at dilution of 1:50. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on KIT. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to KIT. (Distance between topics and target gene indicate popularity.) KIT

* Data provided by citexs.com, for reference only.

Publishing research using A0357? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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