Products > Catalog Antibodies > Polyclonal Antibodies

CD10/MME Rabbit pAb (A5664)

Publications (3) Datasheet

ABclonal:Western blot - CD10/MME Rabbit pAb (A5664)

Western blot analysis of extracts of various cell lines, using CD10/MME antibody (A5664) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - CD10/MME Rabbit pAb (A5664)

Immunohistochemistry of paraffin-embedded human liver using CD10/MME Rabbit pAb (A5664) at dilution of 1:50 (40x lens).

ABclonal:Immunohistochemistry - CD10/MME Rabbit pAb (A5664)

Immunohistochemistry of paraffin-embedded human tonsil using CD10/MME Rabbit pAb (A5664) at dilution of 1:50 (40x lens).

ABclonal:Immunofluorescence - CD10/MME Rabbit pAb (A5664)

Immunofluorescence analysis of human placenta using CD10/CD10/MME antibody (A5664) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameCD10/MME Rabbit pAb
Catalog No.A5664
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 52-352 of human CD10/CD10/MME (NP_009220.2).
SequenceYDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDSRGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYL
Gene ID4311
Swiss protP08473
SynonymsCALLA; CD10; CMT2T; NEP; SCA43; SFE; MME
Calculated MW85kDa
Observed MW100KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, Mouse lung, Mouse kidney, Rat kidney
Cellular locationCell membrane, Single-pass type II membrane protein
Customer validation

WB(Mus musculus, Homo sapiens)

IHC(Mus musculus)

ABclonal:Western blot - CD10/MME Rabbit pAb (A5664) }

Western blot - CD10/MME Rabbit pAb (A5664)

Western blot analysis of extracts of various cell lines, using CD10/MME antibody (A5664) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - CD10/MME Rabbit pAb (A5664) }

Immunohistochemistry - CD10/MME Rabbit pAb (A5664)

Immunohistochemistry of paraffin-embedded human liver using CD10/MME Rabbit pAb (A5664) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - CD10/MME Rabbit pAb (A5664) }

Immunohistochemistry - CD10/MME Rabbit pAb (A5664)

Immunohistochemistry of paraffin-embedded human tonsil using CD10/MME Rabbit pAb (A5664) at dilution of 1:50 (40x lens).
ABclonal:Immunofluorescence - CD10/MME Rabbit pAb (A5664) }

Immunofluorescence - CD10/MME Rabbit pAb (A5664)

Immunofluorescence analysis of human placenta using CD10/CD10/MME antibody (A5664) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Inquire About This Product

Submit your question about A5664 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A5664? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μL sample size available Add to Cart
Contact us to order