Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CD10/MME Rabbit pAb (A5664)

Publications (5) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - CD10/MME Rabbit pAb (A5664)

Western blot analysis of various lysates using CD10/MME Rabbit pAb (A5664) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - CD10/MME Rabbit pAb (A5664)

Immunohistochemistry analysis of CD10/MME in paraffin-embedded human liver using CD10/MME Rabbit pAb (A5664) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CD10/MME Rabbit pAb (A5664)

Immunohistochemistry analysis of CD10/MME in paraffin-embedded human tonsil using CD10/MME Rabbit pAb (A5664) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CD10/MME Rabbit pAb (A5664)

Immunohistochemistry analysis of CD10/MME in paraffin-embedded human tonsil tissue using CD10/MME Rabbit pAb (A5664) at a dilution of 1:50 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

You may also interested in:

Overview

Product name CD10/MME Rabbit pAb
Catalog No. A5664
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 52-352 of human CD10/MME (NP_000893.2).
Sequence YDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDSRGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYL
Gene ID 4311
Swiss prot P08473
Synonyms NEP; SFE; CD10; CALLA; CMT2T; SCA43; CD10/MME
Calculated MW 86kDa
Observed MW 100kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples 293T, Mouse lung, Mouse kidney, Rat kidney
Cellular location Cell membrane, Single-pass type II membrane protein
Customer validation

WB (Mus musculus, Homo sapiens)

IHC (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

CD10/MME Rabbit pAb images

ABclonal:Western blot - CD10/MME Rabbit pAb (A5664)}

Western blot - CD10/MME Rabbit pAb (A5664)

Western blot analysis of various lysates using CD10/MME Rabbit pAb (A5664) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - CD10/MME Rabbit pAb (A5664)}

Immunohistochemistry - CD10/MME Rabbit pAb (A5664)

Immunohistochemistry analysis of CD10/MME in paraffin-embedded human liver using CD10/MME Rabbit pAb (A5664) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CD10/MME Rabbit pAb (A5664)}

Immunohistochemistry - CD10/MME Rabbit pAb (A5664)

Immunohistochemistry analysis of CD10/MME in paraffin-embedded human tonsil using CD10/MME Rabbit pAb (A5664) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CD10/MME Rabbit pAb (A5664)}

Immunohistochemistry - CD10/MME Rabbit pAb (A5664)

Immunohistochemistry analysis of CD10/MME in paraffin-embedded human tonsil tissue using CD10/MME Rabbit pAb (A5664) at a dilution of 1:50 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Inquire About This Product

Submit your question about A5664 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on MME. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MME. (Distance between topics and target gene indicate popularity.) MME

* Data provided by citexs.com, for reference only.

Publishing research using A5664? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order