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CD105/Endoglin Rabbit pAb (A5639)

Publication (1) Datasheet

ABclonal:Western blot - CD105/Endoglin Rabbit pAb (A5639)

Western blot analysis of extracts of various cell lines, using CD105/Endoglin antibody (A5639) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.

Overview

Product nameCD105/Endoglin Rabbit pAb
Catalog No.A5639
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 26-306 of human CD105/Endoglin (NP_001108225.1).
SequenceETVHCDLQPVGPERGEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQASKQNGTWPREVLLVLSVNSSVFLHLQALGIPLHLAYNSSLVTFQEPPGVNTTELPSFPKTQILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVRGCHLEGVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDANHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEARML
Gene ID2022
Swiss protP17813
SynonymsEND; HHT1; ORW1; CD105; ENG; endoglin
Calculated MW67kDa/70kDa
Observed MW71kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samples293T, HL-60, THP-1, HeLa, NCI-H460
Cellular locationMembrane, Single-pass type I membrane protein
Customer validation

FC(Homo sapiens)

ABclonal:Western blot - CD105/Endoglin Rabbit pAb (A5639) }

Western blot - CD105/Endoglin Rabbit pAb (A5639)

Western blot analysis of extracts of various cell lines, using CD105/Endoglin antibody (A5639) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

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