Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CCT7 Rabbit pAb (A12146)

Publications (2) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - CCT7 Rabbit pAb (A12146)

Western blot analysis of various lysates using CCT7 Rabbit pAb (A12146) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Overview

Product name CCT7 Rabbit pAb
Catalog No. A12146
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 6.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 310-500 of human CCT7 (NP_006420.1).
Sequence CAGRVPEEDLKRTMMACGGSIQTSVNALSADVLGRCQVFEETQIGGERYNFFTGCPKAKTCTFILRGGAEQFMEETERSLHDAIMIVRRAIKNDSVVAGGGAIEMELSKYLRDYSRTIPGKQQLLIGAYAKALEIIPRQLCDNAGFDATNILNKLRARHAQGGTWYGVDINNEDIADNFEAFVWEPAMVRI
Gene ID 10574
Swiss prot Q99832
Synonyms CCTH; CCTETA; NIP7-1; TCP1ETA; CCT7
Calculated MW 59kDa
Observed MW 55kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples U-87MG, A-431, HT-29, HeLa, NIH/3T3, Mouse testis, Rat testis
Cellular location Cytoplasm
Customer validation

WB (Rattus norvegicus, Gallus gallus)

Documents

Certificate of Compliance

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Lot number

Research Area

CCT7 Rabbit pAb images

ABclonal:Western blot - CCT7 Rabbit pAb (A12146)}

Western blot - CCT7 Rabbit pAb (A12146)

Western blot analysis of various lysates using CCT7 Rabbit pAb (A12146) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on CCT7. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CCT7. (Distance between topics and target gene indicate popularity.) CCT7

* Data provided by citexs.com, for reference only.

Publishing research using A12146? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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