Review (1)Publications (21) Datasheet SDS
Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat
Western blot analysis of A-549, using CCL2/MCP-1 antibody (A7277) at 1:2000 dilution.A-549 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
Western blot analysis of various lysates, using CCL2/MCP-1 antibody (A7277) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded Rat spleen using CCL2/MCP-1 antibody (A7277) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Mouse spleen using CCL2/MCP-1 antibody (A7277) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human colon carcinoma using CCL2/MCP-1 antibody (A7277) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
| Product name | CCL2/MCP-1 Rabbit pAb |
|---|---|
| Catalog No. | A7277 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 24-99 of human CCL2/MCP-1 (NP_002973.1). |
|---|---|
| Sequence | QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT |
| Gene ID | 6347 |
| Swiss prot | P13500 |
| Synonyms | CCL2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF |
| Calculated MW | 11kDa |
| Observed MW | 13-15KDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us. |
| Application key | Western blotting Immunohistochemistry |
| Positive samples | A-549 |
| Cellular location | Secreted |
| Customer validation | (rat brain cells) WB(Mus musculus, Homo sapiens, Rattus norvegicus) IHC(Rattus norvegicus, Mus musculus) IHC(Mus musculus, Homo sapiens) WB、IHC (Homo sapiens) |
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Based on existing publications, the following genes are closely related to research on CCL2. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to CCL2. (Distance between topics and target gene indicate popularity.) CCL2
* Data provided by citexs.com, for reference only.
Publishing research using A7277? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.
