Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CCL2/MCP-1 Rabbit pAb (A7277)

Review (1)Publications (21) Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - CCL2/MCP-1 Rabbit pAb (A7277)

Western blot analysis of A-549, using CCL2/MCP-1 antibody (A7277) at 1:2000 dilution.A-549 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Western blot - CCL2/MCP-1 Rabbit pAb (A7277)

Western blot analysis of various lysates, using CCL2/MCP-1 antibody (A7277) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Immunohistochemistry - CCL2/MCP-1 Rabbit pAb (A7277)

Immunohistochemistry of paraffin-embedded Rat spleen using CCL2/MCP-1 antibody (A7277) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CCL2/MCP-1 Rabbit pAb (A7277)

Immunohistochemistry of paraffin-embedded Mouse spleen using CCL2/MCP-1 antibody (A7277) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CCL2/MCP-1 Rabbit pAb (A7277)

Immunohistochemistry of paraffin-embedded Human colon carcinoma using CCL2/MCP-1 antibody (A7277) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Review
ABclonal: review for CCL2/MCP-1 Rabbit pAb(A7277)

Overview

Product nameCCL2/MCP-1 Rabbit pAb
Catalog No.A7277
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

Immunogen information

ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-99 of human CCL2/MCP-1 (NP_002973.1).
SequenceQPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT
Gene ID6347
Swiss protP13500
SynonymsCCL2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF
Calculated MW11kDa
Observed MW13-15KDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesA-549
Cellular locationSecreted
Customer validation

(rat brain cells)

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

IHC(Rattus norvegicus, Mus musculus)

IHC(Mus musculus, Homo sapiens)

WB、IHC (Homo sapiens)

Research Area

CCL2/MCP-1 Rabbit pAb images

ABclonal:Western blot - CCL2/MCP-1 Rabbit pAb (A7277)}

Western blot - CCL2/MCP-1 Rabbit pAb (A7277)

Western blot analysis of A-549, using CCL2/MCP-1 antibody (A7277) at 1:2000 dilution.A-549 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Western blot - CCL2/MCP-1 Rabbit pAb (A7277)}

Western blot - CCL2/MCP-1 Rabbit pAb (A7277)

Western blot analysis of various lysates, using CCL2/MCP-1 antibody (A7277) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunohistochemistry - CCL2/MCP-1 Rabbit pAb (A7277)}

Immunohistochemistry - CCL2/MCP-1 Rabbit pAb (A7277)

Immunohistochemistry of paraffin-embedded Rat spleen using CCL2/MCP-1 antibody (A7277) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CCL2/MCP-1 Rabbit pAb (A7277)}

Immunohistochemistry - CCL2/MCP-1 Rabbit pAb (A7277)

Immunohistochemistry of paraffin-embedded Mouse spleen using CCL2/MCP-1 antibody (A7277) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CCL2/MCP-1 Rabbit pAb (A7277)}

Immunohistochemistry - CCL2/MCP-1 Rabbit pAb (A7277)

Immunohistochemistry of paraffin-embedded Human colon carcinoma using CCL2/MCP-1 antibody (A7277) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on CCL2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CCL2. (Distance between topics and target gene indicate popularity.) CCL2

* Data provided by citexs.com, for reference only.

Publishing research using A7277? Please let us know so that we can cite the reference in this datasheet.

Proteins (4)

Primary Antibodies (2)

Secondary Antibodies (26)

* For research use only. Not for therapeutic or diagnostic purposes.

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