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CCDC47 Polyclonal Antibody (A15871)

Datasheet

ABclonal:Western blot - CCDC47 Polyclonal Antibody (A15871)
ABclonal:Immunofluorescence - CCDC47 Polyclonal Antibody (A15871)
ABclonal:Immunofluorescence - CCDC47 Polyclonal Antibody (A15871)
ABclonal:Immunofluorescence - CCDC47 Polyclonal Antibody (A15871)

Overview

Product nameCCDC47 Polyclonal Antibody
Catalog No.A15871
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 224-483 of human CCDC47 (NP_064583.2).
SequenceFLKRQDLLNVLARMMRPVSDQVQIKVTMNDEDMDTYVFAVGTRKALVRLQKEMQDLSEFCSDKPKSGAKYGLPDSLAILSEMGEVTDGMMDTKMVHFLTHYADKIESVHFSDQFSGPKIMQEEGQPLKLPDTKRTLLFTFNVPGSGNTYPKDMEALLPLMNMVIYSIDKAKKFRLNREGKQKADKNRARVEENFLKLTHVQRQEAAQSRREEKKRAEKERIMNEEDPEKQRRLEEAALRREQKKLEKKQMKMKQIKVKAM
Gene ID57003
Swiss protQ96A33
SynonymsCCDC47; GK001; MSTP041; coiled-coil domain containing 47
Calculated MW55kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesLO2, OVCAR3, Mouse lung
Cellular locationMembrane, Single-pass membrane protein
ABclonal:Western blot - CCDC47 Polyclonal Antibody (A15871) }

Western blot - CCDC47 Polyclonal Antibody (A15871)

Western blot analysis of extracts of various cell lines, using CCDC47 antibody (A15871) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunofluorescence - CCDC47 Polyclonal Antibody (A15871) }

Immunofluorescence - CCDC47 Polyclonal Antibody (A15871)

Immunofluorescence analysis of C6 cells using CCDC47 antibody (A15871) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CCDC47 Polyclonal Antibody (A15871) }

Immunofluorescence - CCDC47 Polyclonal Antibody (A15871)

Immunofluorescence analysis of L929 cells using CCDC47 antibody (A15871) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CCDC47 Polyclonal Antibody (A15871) }

Immunofluorescence - CCDC47 Polyclonal Antibody (A15871)

Immunofluorescence analysis of U-2 OS cells using CCDC47 antibody (A15871) at dilution of 1:100. Blue: DAPI for nuclear staining.

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