Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Caspase-6 Rabbit mAb (A12991)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Caspase-6 Rabbit mAb (A12991)

Western blot analysis of various lysates using Caspase-6 Rabbit mAb (A12991) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Overview

Product name Caspase-6 Rabbit mAb
Catalog No. A12991
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms.

Immunogen information

Immunogen A synthetic peptide of human Caspase-6
Sequence Email for sequence
Gene ID 839
Swiss prot P55212
Synonyms MCH2; CSP-6; caspase-6; Caspase-6
Calculated MW 33kDa
Observed MW 38kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Jurkat, NIH/3T3, PC-12, mouse lung, mouse liver, mouse kidney
Cellular location Cytoplasm

Documents

Certificate of Compliance

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Lot number

Research Area

Caspase-6 Rabbit mAb images

ABclonal:Western blot - Caspase-6 Rabbit mAb (A12991)}

Western blot - Caspase-6 Rabbit mAb (A12991)

Western blot analysis of various lysates using Caspase-6 Rabbit mAb (A12991) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on CASP6. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CASP6. (Distance between topics and target gene indicate popularity.) CASP6

* Data provided by citexs.com, for reference only.

Publishing research using A12991? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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