Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

CAPZA2 Rabbit pAb (A2054)

Publication (1) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - CAPZA2 Rabbit pAb (A2054)

Western blot analysis of various lysates using CAPZA2 Rabbit pAb (A2054) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - CAPZA2 Rabbit pAb (A2054)

Immunohistochemistry analysis of CAPZA2 in paraffin-embedded Human colon using CAPZA2 Rabbit pAb (A2054) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CAPZA2 Rabbit pAb (A2054)

Immunohistochemistry analysis of CAPZA2 in paraffin-embedded Mouse kidney using CAPZA2 Rabbit pAb (A2054) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Overview

Product name CAPZA2 Rabbit pAb
Catalog No. A2054
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the F-actin capping protein alpha subunit family. It is the alpha subunit of the barbed-end actin binding protein Cap Z. By capping the barbed end of actin filaments, Cap Z regulates the growth of the actin filaments at the barbed end.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-286 of human CAPZA2 (NP_006127.1).
Sequence MADLEEQLSDEEKVRIAAKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATDPRPCEVENAVESWRTSVETALRAYVKEHYPNGVCTVYGKKIDGQQTIIACIESHQFQAKNFWNGRWRSEWKFTITPSTTQVVGILKIQVHYYEDGNVQLVSHKDIQDSLTVSNEVQTAKEFIKIVEAAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA
Gene ID 830
Swiss prot P47755
Synonyms CAPZ; CAPPA2; CAPZA2
Calculated MW 33kDa
Observed MW 38kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HeLa, THP-1, 22Rv1, Mouse brain, Mouse heart
Cellular location actin cytoskeleton, cortical cytoskeleton, cytosol, extracellular exosome, extracellular region
Customer validation

WB (Rattus norvegicus)

IHC (Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

CAPZA2 Rabbit pAb images

ABclonal:Western blot - CAPZA2 Rabbit pAb (A2054)}

Western blot - CAPZA2 Rabbit pAb (A2054)

Western blot analysis of various lysates using CAPZA2 Rabbit pAb (A2054) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - CAPZA2 Rabbit pAb (A2054)}

Immunohistochemistry - CAPZA2 Rabbit pAb (A2054)

Immunohistochemistry analysis of CAPZA2 in paraffin-embedded Human colon using CAPZA2 Rabbit pAb (A2054) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CAPZA2 Rabbit pAb (A2054)}

Immunohistochemistry - CAPZA2 Rabbit pAb (A2054)

Immunohistochemistry analysis of CAPZA2 in paraffin-embedded Mouse kidney using CAPZA2 Rabbit pAb (A2054) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on CAPZA2. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to CAPZA2. (Distance between topics and target gene indicate popularity.) CAPZA2

* Data provided by citexs.com, for reference only.

Publishing research using A2054? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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