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Carbonic Anhydrase 1 (CA1) Rabbit pAb (A1031)

Datasheet

ABclonal:Western blot - Carbonic Anhydrase 1 (CA1) Rabbit pAb (A1031)

Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase 1 (Carbonic Anhydrase 1 (CA1)) antibody (A1031) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - Carbonic Anhydrase 1 (CA1) Rabbit pAb (A1031)

Immunofluorescence analysis of A-549 cells using Carbonic Anhydrase 1 (Carbonic Anhydrase 1 (CA1)) antibody (A1031). Blue: DAPI for nuclear staining.

Overview

Product nameCarbonic Anhydrase 1 (CA1) Rabbit pAb
Catalog No.A1031
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human Carbonic Anhydrase 1 (Carbonic Anhydrase 1 (CA1)) (NP_001122303.1).
SequenceMASPDWGYDDKNGPEQWSKLYPIANGNNQSPVDIKTSETKHDTSLKPISVSYNPATAKEIINVGHSFHVNFEDNDNRSVLKGGPFSDSYRLFQFHFHWGSTNEHGSEHTVDGVKYSAELHVAHWNSAKYSSLAEAASKADGLAVIGVLMKVGEANPKLQKVLDALQAIKTKGKRAPFTNFDPSTLLPSSLDFWTYPGSLTHPPLYESVTWIICKESISVSSEQLAQFRSLLSNVEGDNAVPMQHNNRPTQPLKGRTVRASF
Gene ID759
Swiss protP00915
SynonymsCA1; CA-I; CAB; Car1; HEL-S-11
Calculated MW28kDa
Observed MW30kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samples293T, Mouse liver, Mouse heart, Mouse brain
Cellular locationCytoplasm
ABclonal:Western blot - Carbonic Anhydrase 1 (CA1) Rabbit pAb (A1031) }

Western blot - Carbonic Anhydrase 1 (CA1) Rabbit pAb (A1031)

Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase 1 (Carbonic Anhydrase 1 (CA1)) antibody (A1031) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - Carbonic Anhydrase 1 (CA1) Rabbit pAb (A1031) }

Immunofluorescence - Carbonic Anhydrase 1 (CA1) Rabbit pAb (A1031)

Immunofluorescence analysis of A-549 cells using Carbonic Anhydrase 1 (Carbonic Anhydrase 1 (CA1)) antibody (A1031). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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