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C6orf25 Polyclonal Antibody (A10154)

Datasheet

ABclonal:Western blot - C6orf25 Polyclonal Antibody (A10154)

Overview

Product nameC6orf25 Polyclonal Antibody
Catalog No.A10154
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the immunoglobulin (Ig) superfamily and is located in the major histocompatibility complex (MHC) class III region. The protein encoded by this gene is a glycosylated, plasma membrane-bound cell surface receptor, but soluble isoforms encoded by some transcript variants have been found in the endoplasmic reticulum and Golgi before being secreted. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 18-142 of human C6orf25 (NP_612121.1).
SequenceNPGASLDGRPGDRVNLSCGGVSHPIRWVWAPSFPACKGLSKGRRPILWASSSGTPTVPPLQPFVGRLRSLDSGIRRLELLLSAGDSGTFFCKGRHEDESRTVLHVLGDRTYCKAPGPTHGSVYPQ
Gene ID80739
Swiss protO95866
SynonymsMPIG6B; C6orf25; G6b; G6b-B; NG31; THAMY; protein G6b
Calculated MW20kDa/21kDa/23kDa/24kDa/25kDa/26kDa
Observed MW26kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:200 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesK-562, Jurkat, SW620, Mouse small intestine, Mouse pancreas, Rat pancreas
Cellular locationCell membrane, Endoplasmic reticulum, Golgi apparatus, Golgi apparatus, Single-pass type I membrane protein
ABclonal:Western blot - C6orf25 Polyclonal Antibody (A10154) }

Western blot - C6orf25 Polyclonal Antibody (A10154)

Western blot analysis of extracts of various cell lines, using C6orf25 antibody (A10154) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

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