Products > Catalog Antibodies > Polyclonal Antibodies

Bcl-2 Rabbit pAb (A0208)

Reviews (7)Publications (94) Datasheet

ABclonal:Bcl-2 Rabbit pAb
ABclonal:Western blot - Bcl-2 Rabbit pAb (A0208)
ABclonal:Immunohistochemistry - Bcl-2 Rabbit pAb (A0208)
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208)
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208)
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208)
ABclonal:Immunoprecipitation - Bcl-2 Rabbit pAb (A0208)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A0208)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A0208)
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ABclonal: review for Bcl-2 Rabbit pAb(A0208)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A0208)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A0208)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A0208)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A0208)

Overview

Product nameBcl-2 Rabbit pAb
Catalog No.A0208
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide of human Bcl-2
SequenceEmail for sequence
Gene ID596
Swiss protP10415
SynonymsBcl-2; PPP1R50; BCL2
Calculated MW22kDa/26kDa
Observed MW26KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293T, THP-1, HeLa
Cellular locationEndoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein
Customer validation

(rat cells, Human skin T cell lymphoma, Hut102, CAL27, B6, MCF-7,MD-MBA-231, HCT116)

WB(Homo sapiens, Rattus norvegicus, Mus musculus, Gallus gallus, Bos taurus, Giardia duodenalis, Capra hircus, Ctenopharyngodon)

IHC(Homo sapiens, Rattus norvegicus, Mus musculus)

IHC(Mus musculus)

IF(Homo sapiens)

ABclonal:Western blot - Bcl-2 Rabbit pAb (A0208) }

Western blot - Bcl-2 Rabbit pAb (A0208)

Western blot analysis of extracts of various cell lines, using Bcl-2 antibody (A0208) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Bcl-2 Rabbit pAb (A0208) }

Immunohistochemistry - Bcl-2 Rabbit pAb (A0208)

Immunohistochemistry of paraffin-embedded human appendix using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208) }

Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of BALB-3T3 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208) }

Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of C6 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208) }

Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of U-2 OS cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Bcl-2 Rabbit pAb (A0208) }

Immunoprecipitation - Bcl-2 Rabbit pAb (A0208)

Immunoprecipitation analysis of 200ug extracts of THP-1 cells using 3ug Bcl-2 antibody (A0208). Western blot was performed from the immunoprecipitate using Bcl-2 antibody (A0208) at a dilition of 1:1000.

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