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BNIP3 Rabbit mAb (A19593)

Publication (1) Datasheet

ABclonal:Western blot - BNIP3 Rabbit mAb (A19593)
ABclonal:Immunohistochemistry - BNIP3 Rabbit mAb (A19593)

Overview

Product nameBNIP3 Rabbit mAb
Catalog No.A19593
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. [provided by RefSeq, Dec 2014]
ImmunogenA synthesized peptide derived from human BNIP3.
SequenceEmail for sequence
Gene ID664
Swiss protQ12983
SynonymsBNIP3; NIP3
Calculated MW30kDa
Observed MW22-28KDa/50-55KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa, NIH/3T3
Cellular locationMitochondrion, Mitochondrion outer membrane, Single-pass membrane protein
Customer validation

WB(Mus musculus)

ABclonal:Western blot - BNIP3 Rabbit mAb (A19593) }

Western blot - BNIP3 Rabbit mAb (A19593)

Western blot analysis of extracts of various cell lines, using BNIP3 antibody (A19593) at 1:1000 dilution.Hela cells and NIH/3T3 cells were treated by Cobalt chloride (0.1 mM) at 37℃ for 4 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - BNIP3 Rabbit mAb (A19593) }

Immunohistochemistry - BNIP3 Rabbit mAb (A19593)

Immunohistochemistry of paraffin-embedded rat heart using BNIP3 antibody (A19593) at dilution of 1:100 (40x lens).

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