Western blot analysis of extracts of various cell lines, using BNIP3 antibody (A19593) at 1:1000 dilution.Hela cells and NIH/3T3 cells were treated by Cobalt chloride (0.1 mM) at 37℃ for 4 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
| Product name | BNIP3 Rabbit mAb |
|---|---|
| Catalog No. | A19593 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthesized peptide derived from human BNIP3. |
|---|---|
| Sequence | Email for sequence |
| Gene ID | 664 |
| Swiss prot | Q12983 |
| Synonyms | BNIP3; NIP3 |
| Calculated MW | 30kDa |
| Observed MW | 22-28KDa/50-55KDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry |
| Positive samples | HeLa, NIH/3T3 |
| Cellular location | Mitochondrion, Mitochondrion outer membrane, Single-pass membrane protein |
| Customer validation | WB(Mus musculus) |
* For research use only. Not for therapeutic or diagnostic purposes.
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