Products > Catalog Antibodies > Monoclonal Antibodies

BNIP3 Rabbit mAb (A19593)

Publication (1) Datasheet

ABclonal:Western blot - BNIP3 Rabbit mAb (A19593)
ABclonal:Immunohistochemistry - BNIP3 Rabbit mAb (A19593)


Product nameBNIP3 Rabbit mAb
Catalog No.A19593
Host speciesRabbit
Purification methodAffinity purification
This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. [provided by RefSeq, Dec 2014]
ImmunogenA synthesized peptide derived from human BNIP3.
SequenceEmail for sequence
Gene ID664
Swiss protQ12983
SynonymsBNIP3; NIP3
Calculated MW30kDa
Observed MW22-28KDa/50-55KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa, NIH/3T3
Cellular locationMitochondrion, Mitochondrion outer membrane, Single-pass membrane protein
Customer validation

WB(Mus musculus)

ABclonal:Western blot - BNIP3 Rabbit mAb (A19593) }

Western blot - BNIP3 Rabbit mAb (A19593)

Western blot analysis of extracts of various cell lines, using BNIP3 antibody (A19593) at 1:1000 dilution.Hela cells and NIH/3T3 cells were treated by Cobalt chloride (0.1 mM) at 37℃ for 4 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - BNIP3 Rabbit mAb (A19593) }

Immunohistochemistry - BNIP3 Rabbit mAb (A19593)

Immunohistochemistry of paraffin-embedded rat heart using BNIP3 antibody (A19593) at dilution of 1:100 (40x lens).

Inquire About This Product

Submit your question about A19593 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A19593? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
Contact us to order