Products > Catalog Antibodies > Polyclonal Antibodies

BNIP3 Rabbit pAb (A5683)

Reviews (2)Publications (2) Datasheet

ABclonal:Western blot - BNIP3 Polyclonal Antibody (A5683)
ABclonal:Immunohistochemistry - BNIP3 Polyclonal Antibody (A5683)
ABclonal:Immunohistochemistry - BNIP3 Polyclonal Antibody (A5683)
ABclonal:Immunohistochemistry - BNIP3 Polyclonal Antibody (A5683)
ABclonal:Immunofluorescence - BNIP3 Polyclonal Antibody (A5683)
Review
ABclonal: review for BNIP3 Rabbit pAb(A5683)
Review
ABclonal: review for BNIP3 Rabbit pAb(A5683)

Overview

Product nameBNIP3 Rabbit pAb
Catalog No.A5683
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human BNIP3 (NP_004043.3).
SequenceMGDAAADPPGPALPCEFLRPGCGAPLSPGAQLGRGAPTSAFPPPAAEAHPAARRGLRSPQLPSGAMSQNGAPGMQEESLQGSWVELHFSNNGNGGSVPASVSIYNGDMEKILLDAQHESGRSSSKSSHCDSPPRSQTPQDTNRASETDTHSIGEKNSSQSEEDD
Gene ID664
Swiss protQ12983
SynonymsBNIP3; NIP3
Calculated MW27kDa
Observed MW22-70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7, NCI-H460, Jurkat, SH-SY5Y, Mouse kidney, Mouse heart, Mouse liver, Rat brain
Cellular locationMitochondrion, Mitochondrion outer membrane, Single-pass membrane protein
Customer validation

(Mouse heart, Human Isikawa )

IHC(Mus musculus)

WB(Homo sapiens)

ABclonal:Western blot - BNIP3 Polyclonal Antibody (A5683) }

Western blot - BNIP3 Polyclonal Antibody (A5683)

Western blot analysis of extracts of various cell lines, using BNIP3 antibody (A5683) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunohistochemistry - BNIP3 Polyclonal Antibody (A5683) }

Immunohistochemistry - BNIP3 Polyclonal Antibody (A5683)

Immunohistochemistry of paraffin-embedded mouse kidney using BNIP3 antibody (A5683) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - BNIP3 Polyclonal Antibody (A5683) }

Immunohistochemistry - BNIP3 Polyclonal Antibody (A5683)

Immunohistochemistry of paraffin-embedded rat heart using BNIP3 antibody (A5683) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - BNIP3 Polyclonal Antibody (A5683) }

Immunohistochemistry - BNIP3 Polyclonal Antibody (A5683)

Immunohistochemistry of paraffin-embedded human liver damage using BNIP3 antibody (A5683) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - BNIP3 Polyclonal Antibody (A5683) }

Immunofluorescence - BNIP3 Polyclonal Antibody (A5683)

Immunofluorescence analysis of HeLa cells using BNIP3 antibody (A5683) at dilution of 1:100. Blue: DAPI for nuclear staining.

Submit an review for A5683

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A5683 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A5683? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μl sample size available Add to Cart
Contact us to order