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BNIP3 Polyclonal Antibody (A5683)

Review (2)Publication (2) Datasheet

ABclonal:Western blot - BNIP3 Polyclonal Antibody (A5683)
ABclonal:Immunohistochemistry - BNIP3 Polyclonal Antibody (A5683)
ABclonal:Immunohistochemistry - BNIP3 Polyclonal Antibody (A5683)
ABclonal:Immunohistochemistry - BNIP3 Polyclonal Antibody (A5683)
ABclonal:Immunofluorescence - BNIP3 Polyclonal Antibody (A5683)
Review
ABclonal: review for BNIP3 Polyclonal Antibody(A5683)
Review
ABclonal: review for BNIP3 Polyclonal Antibody(A5683)

Overview

Product nameBNIP3 Polyclonal Antibody
Catalog No.A5683
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human BNIP3 (NP_004043.3).
SequenceMGDAAADPPGPALPCEFLRPGCGAPLSPGAQLGRGAPTSAFPPPAAEAHPAARRGLRSPQLPSGAMSQNGAPGMQEESLQGSWVELHFSNNGNGGSVPASVSIYNGDMEKILLDAQHESGRSSSKSSHCDSPPRSQTPQDTNRASETDTHSIGEKNSSQSEEDD
Gene ID664
Swiss protQ12983
SynonymsBNIP3; NIP3; BCL2 interacting protein 3
Calculated MW27kDa
Observed MW22-70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7, NCI-H460, Jurkat, SH-SY5Y, Mouse kidney, Mouse heart, Mouse liver, Rat brain
Cellular locationMitochondrion, Mitochondrion outer membrane, Single-pass membrane protein
Customer validation

(, )

IHC(Mouse)

WB(Human)

ABclonal:Western blot - BNIP3 Polyclonal Antibody (A5683) }

Western blot - BNIP3 Polyclonal Antibody (A5683)

Western blot analysis of extracts of various cell lines, using BNIP3 antibody (A5683) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunohistochemistry - BNIP3 Polyclonal Antibody (A5683) }

Immunohistochemistry - BNIP3 Polyclonal Antibody (A5683)

Immunohistochemistry of paraffin-embedded mouse kidney using BNIP3 antibody (A5683) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - BNIP3 Polyclonal Antibody (A5683) }

Immunohistochemistry - BNIP3 Polyclonal Antibody (A5683)

Immunohistochemistry of paraffin-embedded rat heart using BNIP3 antibody (A5683) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - BNIP3 Polyclonal Antibody (A5683) }

Immunohistochemistry - BNIP3 Polyclonal Antibody (A5683)

Immunohistochemistry of paraffin-embedded human liver damage using BNIP3 antibody (A5683) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - BNIP3 Polyclonal Antibody (A5683) }

Immunofluorescence - BNIP3 Polyclonal Antibody (A5683)

Immunofluorescence analysis of HeLa cells using BNIP3 antibody (A5683) at dilution of 1:100. Blue: DAPI for nuclear staining.

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