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BNIP3 Rabbit pAb (A5683)

Reviews (2)Publications (8) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - BNIP3 Rabbit pAb (A5683)

Western blot analysis of extracts of various cell lines, using BNIP3 antibody (A5683) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - BNIP3 Rabbit pAb (A5683)

Immunohistochemistry of paraffin-embedded mouse stomach using BNIP3 Rabbit pAb (A5683) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - BNIP3 Rabbit pAb (A5683)

Immunohistochemistry of paraffin-embedded rat stomach using BNIP3 Rabbit pAb (A5683) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - BNIP3 Rabbit pAb (A5683)

Immunofluorescence analysis of HeLa cells using BNIP3 antibody (A5683) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for BNIP3 Rabbit pAb(A5683)
Review
ABclonal: review for BNIP3 Rabbit pAb(A5683)

Overview

Product nameBNIP3 Rabbit pAb
Catalog No.A5683
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 40-120 of human BNIP3 (NP_004043.3).
SequenceAFPPPAAEAHPAARRGLRSPQLPSGAMSQNGAPGMQEESLQGSWVELHFSNNGNGGSVPASVSIYNGDMEKILLDAQHESG
Gene ID664
Swiss protQ12983
SynonymsBNIP3; NIP3
Calculated MW27kDa
Observed MW22-70KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, NIH/3T3, Rat heart
Cellular locationMitochondrion, Mitochondrion outer membrane, Single-pass membrane protein
Customer validation

(Mouse heart, Human Isikawa )

IHC(Mus musculus)

WB(Homo sapiens, Mus musculus, Rattus norvegicus)

IF(Mus musculus)

IHC(Mus musculus)

IF(Mus musculus)

IF(Rattus norvegicus)

ABclonal:Western blot - BNIP3 Rabbit pAb (A5683)}

Western blot - BNIP3 Rabbit pAb (A5683)

Western blot analysis of extracts of various cell lines, using BNIP3 antibody (A5683) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - BNIP3 Rabbit pAb (A5683)}

Immunohistochemistry - BNIP3 Rabbit pAb (A5683)

Immunohistochemistry of paraffin-embedded mouse stomach using BNIP3 Rabbit pAb (A5683) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - BNIP3 Rabbit pAb (A5683)}

Immunohistochemistry - BNIP3 Rabbit pAb (A5683)

Immunohistochemistry of paraffin-embedded rat stomach using BNIP3 Rabbit pAb (A5683) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - BNIP3 Rabbit pAb (A5683)}

Immunofluorescence - BNIP3 Rabbit pAb (A5683)

Immunofluorescence analysis of HeLa cells using BNIP3 antibody (A5683) at dilution of 1:100. Blue: DAPI for nuclear staining.

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