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BNIP3L Rabbit pAb (A6283)

Review (1)Publication (1) Datasheet

ABclonal:Western blot - BNIP3L Polyclonal Antibody (A6283)
ABclonal:Immunohistochemistry - BNIP3L Polyclonal Antibody (A6283)
ABclonal:Immunohistochemistry - BNIP3L Polyclonal Antibody (A6283)
ABclonal:Immunohistochemistry - BNIP3L Polyclonal Antibody (A6283)
Review
ABclonal: review for BNIP3L Rabbit pAb(A6283)

Overview

Product nameBNIP3L Rabbit pAb
Catalog No.A6283
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that belongs to the pro-apoptotic subfamily within the Bcl-2 family of proteins. The encoded protein binds to Bcl-2 and possesses the BH3 domain. The protein directly targets mitochondria and causes apoptotic changes, including loss of membrane potential and the release of cytochrome c.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human BNIP3L (NP_004322.1).
SequenceMSSHLVEPPPPLHNNNNNCEENEQSLPPPAGLNSSWVELPMNSSNGNDNGNGKNGGLEHVPSSSSIHNGDMEKILLDAQHESGQSSSRGSSHCDSPSPQEDGQIMFDVEMHTSRDHSSQSEEEVVEGEKEVEALKKSADWVSDWSSRPENIPPKEFHFRHPKRSVSLSMRKSGAMKKGGIFSAEFLK
Gene ID665
Swiss protO60238
SynonymsBNIP3L; BNIP3a; NIX
Calculated MW19kDa/23kDa
Observed MW30kDa/45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesSW480, A-549, MCF7, Raji, Mouse spleen, Mouse thymus, Mouse brain, Mouse kidney
Cellular locationEndoplasmic reticulum, Membrane, Mitochondrion outer membrane, Nucleus envelope, Single-pass membrane protein
Customer validation

(Mouse heart)

WB(Mus musculus)

ABclonal:Western blot - BNIP3L Polyclonal Antibody (A6283) }

Western blot - BNIP3L Polyclonal Antibody (A6283)

Western blot analysis of extracts of various cell lines, using BNIP3L antibody (A6283) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunohistochemistry - BNIP3L Polyclonal Antibody (A6283) }

Immunohistochemistry - BNIP3L Polyclonal Antibody (A6283)

Immunohistochemistry of paraffin-embedded human stomach using BNIP3L antibody (A6283) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - BNIP3L Polyclonal Antibody (A6283) }

Immunohistochemistry - BNIP3L Polyclonal Antibody (A6283)

Immunohistochemistry of paraffin-embedded rat brain using BNIP3L antibody (A6283) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - BNIP3L Polyclonal Antibody (A6283) }

Immunohistochemistry - BNIP3L Polyclonal Antibody (A6283)

Immunohistochemistry of paraffin-embedded human lung cancer using BNIP3L antibody (A6283) at dilution of 1:100 (40x lens).

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