Review (1)Publication (1) Datasheet
Western blot analysis of extracts of various cell lines, using BNIP3L antibody (A6283) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse lung using BNIP3L Rabbit pAb (A6283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using BNIP3L Rabbit pAb (A6283) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using BNIP3L Rabbit pAb (A6283) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
| Product name | BNIP3L Rabbit pAb |
|---|---|
| Catalog No. | A6283 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human BNIP3L (NP_004322.1). |
|---|---|
| Sequence | MSSHLVEPPPPLHNNNNNCEENEQSLPPPAGLNSSWVELPMNSSNGNDNGNGKNGGLEHVPSSSSIHNGDMEKILLDAQHESGQSSSRGSSHCDSPSPQEDGQIMFDVEMHTSRDHSSQSEEEVVEGEKEVEALKKSADWVSDWSSRPENIPPKEFHFRHPKRSVSLSMRKSGAMKKGGIFSAEFLK |
| Gene ID | 665 |
| Swiss prot | O60238 |
| Synonyms | BNIP3L; BNIP3a; NIX |
| Calculated MW | 19kDa/23kDa |
| Observed MW | 30kDa/45kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | SW480, A-549, MCF7, Raji, Mouse spleen, Mouse thymus, Mouse brain, Mouse kidney |
| Cellular location | Endoplasmic reticulum, Membrane, Mitochondrion outer membrane, Nucleus envelope, Single-pass membrane protein |
| Customer validation | (Mouse heart) WB(Mus musculus) |
* For research use only. Not for therapeutic or diagnostic purposes.
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