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BNIP3L Rabbit pAb (A6283)

Review (1)Publication (1) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - BNIP3L Rabbit pAb (A6283)

Western blot analysis of extracts of various cell lines, using BNIP3L antibody (A6283) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - BNIP3L Rabbit pAb (A6283)

Immunohistochemistry of paraffin-embedded mouse lung using BNIP3L Rabbit pAb (A6283) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - BNIP3L Rabbit pAb (A6283)

Immunohistochemistry of paraffin-embedded rat kidney using BNIP3L Rabbit pAb (A6283) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - BNIP3L Rabbit pAb (A6283)

Immunofluorescence analysis of NIH/3T3 cells using BNIP3L Rabbit pAb (A6283) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for BNIP3L Rabbit pAb(A6283)

Overview

Product nameBNIP3L Rabbit pAb
Catalog No.A6283
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that belongs to the pro-apoptotic subfamily within the Bcl-2 family of proteins. The encoded protein binds to Bcl-2 and possesses the BH3 domain. The protein directly targets mitochondria and causes apoptotic changes, including loss of membrane potential and the release of cytochrome c.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human BNIP3L (NP_004322.1).
SequenceMSSHLVEPPPPLHNNNNNCEENEQSLPPPAGLNSSWVELPMNSSNGNDNGNGKNGGLEHVPSSSSIHNGDMEKILLDAQHESGQSSSRGSSHCDSPSPQEDGQIMFDVEMHTSRDHSSQSEEEVVEGEKEVEALKKSADWVSDWSSRPENIPPKEFHFRHPKRSVSLSMRKSGAMKKGGIFSAEFLK
Gene ID665
Swiss protO60238
SynonymsBNIP3L; BNIP3a; NIX
Calculated MW19kDa/23kDa
Observed MW45KDa/70KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Rat kidney
Cellular locationEndoplasmic reticulum, Membrane, Mitochondrion outer membrane, Nucleus envelope, Single-pass membrane protein
Customer validation

(Mouse heart)

WB(Mus musculus)

ABclonal:Western blot - BNIP3L Rabbit pAb (A6283) }

Western blot - BNIP3L Rabbit pAb (A6283)

Western blot analysis of extracts of various cell lines, using BNIP3L antibody (A6283) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - BNIP3L Rabbit pAb (A6283) }

Immunohistochemistry - BNIP3L Rabbit pAb (A6283)

Immunohistochemistry of paraffin-embedded mouse lung using BNIP3L Rabbit pAb (A6283) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - BNIP3L Rabbit pAb (A6283) }

Immunohistochemistry - BNIP3L Rabbit pAb (A6283)

Immunohistochemistry of paraffin-embedded rat kidney using BNIP3L Rabbit pAb (A6283) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - BNIP3L Rabbit pAb (A6283) }

Immunofluorescence - BNIP3L Rabbit pAb (A6283)

Immunofluorescence analysis of NIH/3T3 cells using BNIP3L Rabbit pAb (A6283) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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