Products > Catalog Antibodies > Polyclonal Antibodies

BNIP3L Rabbit pAb (A6283)

Tested applications:WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human, Mouse, Rat

Western blot analysis of extracts of various cell lines, using BNIP3L antibody (A6283) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - BNIP3L Rabbit pAb (A6283)

Immunohistochemistry of paraffin-embedded mouse lung using BNIP3L Rabbit pAb (A6283) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using BNIP3L Rabbit pAb (A6283) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - BNIP3L Rabbit pAb (A6283)

Immunofluorescence analysis of NIH/3T3 cells using BNIP3L Rabbit pAb (A6283) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review

Overview

Product name	BNIP3L Rabbit pAb
Catalog No.	A6283
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a protein that belongs to the pro-apoptotic subfamily within the Bcl-2 family of proteins. The encoded protein binds to Bcl-2 and possesses the BH3 domain. The protein directly targets mitochondria and causes apoptotic changes, including loss of membrane potential and the release of cytochrome c.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human BNIP3L (NP_004322.1).
Sequence	MSSHLVEPPPPLHNNNNNCEENEQSLPPPAGLNSSWVELPMNSSNGNDNGNGKNGGLEHVPSSSSIHNGDMEKILLDAQHESGQSSSRGSSHCDSPSPQEDGQIMFDVEMHTSRDHSSQSEEEVVEGEKEVEALKKSADWVSDWSSRPENIPPKEFHFRHPKRSVSLSMRKSGAMKKGGIFSAEFLK
Gene ID	665
Swiss prot	O60238
Synonyms	BNIP3L; BNIP3a; NIX
Calculated MW	19kDa/23kDa
Observed MW	45KDa/70KDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa, Rat kidney
Cellular location	Endoplasmic reticulum, Membrane, Mitochondrion outer membrane, Nucleus envelope, Single-pass membrane protein
Customer validation	(Mouse heart) WB(Mus musculus)