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BMP2 Rabbit pAb (A0231)

Publications (3) Datasheet

ABclonal:Western blot - BMP2 Rabbit pAb (A0231)

Western blot analysis of extracts of various cell lines, using BMP2 antibody (A0231) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - BMP2 Rabbit pAb (A0231)

Immunohistochemistry of paraffin-embedded human colon using BMP2 antibody (A0231) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - BMP2 Rabbit pAb (A0231)

Immunohistochemistry of paraffin-embedded mouse lung using BMP2 antibody (A0231) at dilution of 1:200 (40x lens).

Overview

Product nameBMP2 Rabbit pAb
Catalog No.A0231
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 283-396 of human BMP2 (NP_001191.1).
SequenceQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR
Gene ID650
Swiss protP12643
SynonymsBDA2; BMP2A; BMP2
Calculated MW44kDa
Observed MW45KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHT-29, Mouse large intestine, Mouse stomach, Rat liver
Cellular locationSecreted
Customer validation

IHC(Oryctolagus cuniculus)

WB(Homo sapiens, Gallus gallus)

IHC(Homo sapiens, Gallus gallus)

ABclonal:Western blot - BMP2 Rabbit pAb (A0231) }

Western blot - BMP2 Rabbit pAb (A0231)

Western blot analysis of extracts of various cell lines, using BMP2 antibody (A0231) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - BMP2 Rabbit pAb (A0231) }

Immunohistochemistry - BMP2 Rabbit pAb (A0231)

Immunohistochemistry of paraffin-embedded human colon using BMP2 antibody (A0231) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - BMP2 Rabbit pAb (A0231) }

Immunohistochemistry - BMP2 Rabbit pAb (A0231)

Immunohistochemistry of paraffin-embedded mouse lung using BMP2 antibody (A0231) at dilution of 1:200 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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