Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

BIN2 Rabbit pAb (A21175)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Arabidopsis thaliana

ABclonal:Western blot - BIN2 Rabbit pAb (A21175)

Western blot analysis of lysates from Rosette leaves, using BIN2 Rabbit pAb (A21175) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Overview

Product name BIN2 Rabbit pAb
Catalog No. A21175
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Encodes BIN2, a member of the ATSK (shaggy-like kinase) family. BIN2 functions in the cross-talk between auxin and brassinosteroid signaling pathways. BIN2 regulates root epidermal cell fate specification by phosphorylating EGL3 and TTG1. BIN2-mediated phosphorylation appears to promote BZR1 export from the nucleus.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 281-380 of arabidopsis thaliana BIN2 (NP_193606.1).
Sequence KAHPWHKIFHKRMPPEAIDFASRLLQYSPSLRCTALEACAHPFFDELREPNARLPNGRPFPPLFNFKQEVAGSSPELVNKLIPDHIKRQLGLSFLNQSGT
Gene ID 827605
Swiss prot Q39011
Synonyms ATSK21; BRASSINOSTEROID-INSENSITIVE 2; DWARF 12; DWF12; F28A21.120; F28A21_120; SHAGGY-LIKE KINASE 21; SK21; UCU1; ULTRACURVATA 1; BIN2
Calculated MW 43kDa
Observed MW 43kDa

Applications

Reactivity Arabidopsis thaliana
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Rosette leaves
Cellular location cell cortex, cytoplasm, nucleus, plasma membrane

Documents

Certificate of Compliance

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Lot number

BIN2 Rabbit pAb images

ABclonal:Western blot - BIN2 Rabbit pAb (A21175)}

Western blot - BIN2 Rabbit pAb (A21175)

Western blot analysis of lysates from Rosette leaves, using BIN2 Rabbit pAb (A21175) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Related Targets

Based on existing publications, the following genes are closely related to research on BIN2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to BIN2. (Distance between topics and target gene indicate popularity.) BIN2

* Data provided by citexs.com, for reference only.

Publishing research using A21175? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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