Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

BDNF Rabbit mAb (A10760)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - BDNF Rabbit mAb (A10760)

Western blot analysis of extracts of various cell lines, using BDNF antibody (A10760).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name BDNF Rabbit mAb
Catalog No. A10760
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders.

Immunogen information

Immunogen Recombinant protein of human BDNF
Sequence Email for sequence
Gene ID 627
Swiss prot P23560
Synonyms ANON2; BULN2; BDNF
Calculated MW 28kDa
Observed MW 28kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples SHG-44, A172, Mouse brain
Cellular location Secreted

Research Area

BDNF Rabbit mAb images

ABclonal:Western blot - BDNF Rabbit mAb (A10760)}

Western blot - BDNF Rabbit mAb (A10760)

Western blot analysis of extracts of various cell lines, using BDNF antibody (A10760).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on BDNF. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to BDNF. (Distance between topics and target gene indicate popularity.) BDNF

* Data provided by citexs.com, for reference only.

Publishing research using A10760? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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