Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

BCLAF1 Rabbit pAb (A16473)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - BCLAF1 Rabbit pAb (A16473)

Western blot analysis of extracts of SH-SY5Y cells, using BCLAF1 antibody (A16473) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Overview

Product name BCLAF1 Rabbit pAb
Catalog No. A16473
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 330-552 of human BCLAF1 (NP_001070909.1).
Sequence TAKTGKFLKSPPLHKNLDAREKSTFREESPLRIKMIASDSHRPEVKLKMAPVPLDDSNRPASLTKDRLLASTLVHSVKKEQEFRSIFDHIKLPQASKSTSESFIQHIVSLVHHVKEQYFKSAAMTLNERFTSYQKATEEHSTRQKSPEIHRRIDISPSTLRKHTRLAGEERVFKEENQKGDKKLRCDSADLRHDIDRRRKERSKERGDSKGSRESSGSRKQEK
Gene ID 9774
Swiss prot Q9NYF8
Synonyms BTF; bK211L9.1; BCLAF1
Calculated MW 106kDa
Observed MW 150kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples SH-SY5Y
Cellular location Cytoplasm, Nucleus, Nucleus speckle, nucleoplasm

Documents

Certificate of Compliance

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Research Area

BCLAF1 Rabbit pAb images

ABclonal:Western blot - BCLAF1 Rabbit pAb (A16473)}

Western blot - BCLAF1 Rabbit pAb (A16473)

Western blot analysis of extracts of SH-SY5Y cells, using BCLAF1 antibody (A16473) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Related Targets

Based on existing publications, the following genes are closely related to research on BCLAF1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to BCLAF1. (Distance between topics and target gene indicate popularity.) BCLAF1

* Data provided by citexs.com, for reference only.

Publishing research using A16473? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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