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BCL2 Polyclonal Antibody (A0208)

Reviews (6)Publications (17) Datasheet

ABclonal:Western blot - BCL2 Polyclonal Antibody (A0208)
ABclonal:Immunohistochemistry - BCL2 Polyclonal Antibody (A0208)
ABclonal:Immunohistochemistry - BCL2 Polyclonal Antibody (A0208)

Overview

Product nameBCL2 Polyclonal Antibody
Catalog No.A0208
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide of human BCL2
SequenceEmail for sequence
Gene ID596
Swiss protP10415
SynonymsBcl-2; PPP1R50
Calculated MW22kDa/26kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHL-60
Cellular locationEndoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein
Customer validation

WB(Human skin T cell lymphoma, CAL27, Hut102, HCT116, MCF-7,MD-MBA-231, B6, Rat, Human, Mouse, chicken)

IHC(Human)

ABclonal:Western blot - BCL2 Polyclonal Antibody (A0208) }

Western blot - BCL2 Polyclonal Antibody (A0208)

Western blot analysis of extracts of HL-60 cells, using BCL2 antibody (A0208) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - BCL2 Polyclonal Antibody (A0208) }

Immunohistochemistry - BCL2 Polyclonal Antibody (A0208)

Immunohistochemistry of paraffin-embedded human liver cancer using BCL2 antibody (A0208) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - BCL2 Polyclonal Antibody (A0208) }

Immunohistochemistry - BCL2 Polyclonal Antibody (A0208)

Immunohistochemistry of paraffin-embedded human gastric cancer using BCL2 antibody (A0208) at dilution of 1:100 (40x lens).

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