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BAX Monoclonal Antibody (A2211)

Publications (2) Datasheet

ABclonal:Western blot - BAX antibody (A2211)

Overview

Product nameBAX Monoclonal Antibody
Catalog No.A2211
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
ImmunogenRecombinant protein of human BAX
SequenceEmail for sequence
Gene ID581
Swiss protQ07812
SynonymsBCL2L4
Calculated MW4kDa/12kDa/15kDa/18kDa/19kDa/21kDa/24kDa
Observed MW21kDa
ReactivityHuman, Monkey
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesCOS7, Raji, HeLa
Cellular locationCytoplasm, Cytoplasm, Mitochondrion membrane, Single-pass membrane protein
Customer validation

WB(Human, Human, Mouse)

ABclonal:Western blot - BAX antibody (A2211)}

Western blot - BAX antibody (A2211)

Western blot analysis of extracts of various cell lines, using BAX antibody (A2211) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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