Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Bad Rabbit mAb (A10753)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Bad Rabbit mAb (A10753)

Western blot analysis of lysates from HeLa cells, using Bad Rabbit mAb (A10753).

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Overview

Product name Bad Rabbit mAb
Catalog No. A10753
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL (B-cell lymphoma-extra large) and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Immunogen information

Immunogen A synthetic peptide of human Bad
Sequence Email for sequence
Gene ID 572
Swiss prot Q92934
Synonyms BBC2; BCL2L8; Bad
Calculated MW 18kDa
Observed MW 27kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
  • IP 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples HeLa
Cellular location Cytoplasm, Mitochondrion outer membrane
Customer validation

WB (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Bad Rabbit mAb images

ABclonal:Western blot - Bad Rabbit mAb (A10753)}

Western blot - Bad Rabbit mAb (A10753)

Western blot analysis of lysates from HeLa cells, using Bad Rabbit mAb (A10753).

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Related Targets

Based on existing publications, the following genes are closely related to research on BAD. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to BAD. (Distance between topics and target gene indicate popularity.) BAD

* Data provided by citexs.com, for reference only.

Publishing research using A10753? Please let us know so that we can cite the reference in this datasheet.

Antibodies (8)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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