Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Aromatase (CYP19A1) Rabbit pAb (A2161)

Publications (12) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Aromatase (CYP19A1) Rabbit pAb (A2161)

Western blot analysis of various lysates using Aromatase (CYP19A1) Rabbit pAb (A2161) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - Aromatase (CYP19A1) Rabbit pAb (A2161)

Immunohistochemistry analysis of Aromatase (CYP19A1) in paraffin-embedded human placenta using Aromatase (CYP19A1) Rabbit pAb (A2161) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Aromatase (CYP19A1) Rabbit pAb (A2161)

Immunohistochemistry analysis of Aromatase (CYP19A1) in paraffin-embedded rat ovary using Aromatase (CYP19A1) Rabbit pAb (A2161) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Aromatase (CYP19A1) Rabbit pAb (A2161)

Immunohistochemistry analysis of Aromatase (CYP19A1) in paraffin-embedded mouse brain using Aromatase (CYP19A1) Rabbit pAb (A2161) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

You may also interested in:

Overview

Product name Aromatase (CYP19A1) Rabbit pAb
Catalog No. A2161
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 234-503 of human Aromatase (CYP19A1) (NP_000094.2).
Sequence FFKISWLYKKYEKSVKDLKDAIEVLIAEKRRRISTEEKLEECMDFATELILAEKRGDLTRENVNQCILEMLIAAPDTMSVSLFFMLFLIAKHPNVEEAIIKEIQTVIGERDIKIDDIQKLKVMENFIYESMRYQPVVDLVMRKALEDDVIDGYPVKKGTNIILNIGRMHRLEFFPKPNEFTLENFAKNVPYRYFQPFGFGPRGCAGKYIAMVMMKAILVTLLRRFHVKTLQGQCVESIQKIHDLSLHPDETKNMLEMIFTPRNSDRCLEH
Gene ID 1588
Swiss prot P11511
Synonyms ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM; Aromatase (CYP19A1)
Calculated MW 58kDa
Observed MW 53kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HepG2, Mouse brain, Mouse intestine
Cellular location Membrane, Peripheral membrane protein
Customer validation

WB (Sus scrofa, Mus musculus, Capra hircus, Homo sapiens)

IHC (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Aromatase (CYP19A1) Rabbit pAb images

ABclonal:Western blot - Aromatase (CYP19A1) Rabbit pAb (A2161)}

Western blot - Aromatase (CYP19A1) Rabbit pAb (A2161)

Western blot analysis of various lysates using Aromatase (CYP19A1) Rabbit pAb (A2161) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - Aromatase (CYP19A1) Rabbit pAb (A2161)}

Immunohistochemistry - Aromatase (CYP19A1) Rabbit pAb (A2161)

Immunohistochemistry analysis of Aromatase (CYP19A1) in paraffin-embedded human placenta using Aromatase (CYP19A1) Rabbit pAb (A2161) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Aromatase (CYP19A1) Rabbit pAb (A2161)}

Immunohistochemistry - Aromatase (CYP19A1) Rabbit pAb (A2161)

Immunohistochemistry analysis of Aromatase (CYP19A1) in paraffin-embedded rat ovary using Aromatase (CYP19A1) Rabbit pAb (A2161) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Aromatase (CYP19A1) Rabbit pAb (A2161)}

Immunohistochemistry - Aromatase (CYP19A1) Rabbit pAb (A2161)

Immunohistochemistry analysis of Aromatase (CYP19A1) in paraffin-embedded mouse brain using Aromatase (CYP19A1) Rabbit pAb (A2161) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Inquire About This Product

Submit your question about A2161 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on CYP19A1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CYP19A1. (Distance between topics and target gene indicate popularity.) CYP19A1

* Data provided by citexs.com, for reference only.

Publishing research using A2161? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

!OUT OF STOCK

See Below for Alternatives
Contact us to order