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Aromatase (CYP19A1) Rabbit pAb (A2161)

Publications (6) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - Aromatase (CYP19A1) Rabbit pAb (A2161)

Western blot analysis of extracts of various cell lines, using Aromatase (CYP19A1) antibody (A2161) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - Aromatase (CYP19A1) Rabbit pAb (A2161)

Immunohistochemistry of paraffin-embedded human placenta using Aromatase (CYP19A1) antibody (A2161) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Aromatase (CYP19A1) Rabbit pAb (A2161)

Immunohistochemistry of paraffin-embedded rat ovary using Aromatase (CYP19A1) antibody (A2161) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Aromatase (CYP19A1) Rabbit pAb (A2161)

Immunohistochemistry of paraffin-embedded mouse brain using Aromatase (CYP19A1) antibody (A2161) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameAromatase (CYP19A1) Rabbit pAb
Catalog No.A2161
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 234-503 of human Aromatase (CYP19A1) (NP_000094.2).
SequenceFFKISWLYKKYEKSVKDLKDAIEVLIAEKRRRISTEEKLEECMDFATELILAEKRGDLTRENVNQCILEMLIAAPDTMSVSLFFMLFLIAKHPNVEEAIIKEIQTVIGERDIKIDDIQKLKVMENFIYESMRYQPVVDLVMRKALEDDVIDGYPVKKGTNIILNIGRMHRLEFFPKPNEFTLENFAKNVPYRYFQPFGFGPRGCAGKYIAMVMMKAILVTLLRRFHVKTLQGQCVESIQKIHDLSLHPDETKNMLEMIFTPRNSDRCLEH
Gene ID1588
Swiss protP11511
SynonymsCYP19A1; ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM; aromatase; Aromatase
Calculated MW24kDa/57kDa
Observed MW53kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHepG2, Mouse brain, Mouse intestine
Cellular locationMembrane, Peripheral membrane protein
Customer validation

WB(Sus scrofa, Mus musculus, Capra hircus, Homo sapiens)

IHC(Mus musculus)

ABclonal:Western blot - Aromatase (CYP19A1) Rabbit pAb (A2161)}

Western blot - Aromatase (CYP19A1) Rabbit pAb (A2161)

Western blot analysis of extracts of various cell lines, using Aromatase (CYP19A1) antibody (A2161) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - Aromatase (CYP19A1) Rabbit pAb (A2161)}

Immunohistochemistry - Aromatase (CYP19A1) Rabbit pAb (A2161)

Immunohistochemistry of paraffin-embedded human placenta using Aromatase (CYP19A1) antibody (A2161) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Aromatase (CYP19A1) Rabbit pAb (A2161)}

Immunohistochemistry - Aromatase (CYP19A1) Rabbit pAb (A2161)

Immunohistochemistry of paraffin-embedded rat ovary using Aromatase (CYP19A1) antibody (A2161) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Aromatase (CYP19A1) Rabbit pAb (A2161)}

Immunohistochemistry - Aromatase (CYP19A1) Rabbit pAb (A2161)

Immunohistochemistry of paraffin-embedded mouse brain using Aromatase (CYP19A1) antibody (A2161) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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