Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

ApoB Rabbit pAb (A1330)

Publications (5) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - ApoB Rabbit pAb (A1330)

Western blot analysis of various lysates using ApoB Rabbit pAb (A1330) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - ApoB Rabbit pAb (A1330)

Immunofluorescence analysis of mouse liver cells using ApoB Rabbit pAb (A1330) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name ApoB Rabbit pAb
Catalog No. A1330
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins (LDL), and is the ligand for the LDL receptor. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 28-330 of human ApoB (NP_000375.3).
Sequence NSLNIAGLSLDFSSKLDNIYSSDKFYKQTVNLQLQPYSLVTTLNSDLKYNALDLTNNGKLRLEPLKLHVAGNLKGAYQNNEIKHIYAISSAALSASYKADTVAKVQGVEFSHRLNTDIAGLASAIDMSTNYNSDSLHFSNVFRSVMAPFTMTIDAHTNGNGKLALWGEHTGQLYSKFLLKAEPLAFTFSHDYKGSTSHHLVSRKSISAALEHKVSALLTPAEQTGTWKLKTQFNNNEYSQDLDAYNTKDKIGVELTGRTLADLTLLDSPIKVPLLLSEPINIIDALEMRDAVEKPQEFTIVAF
Gene ID 338
Swiss prot P04114
Synonyms FLDB; FCHL2; LDLCQ4; apoB-48; apoB-100; ApoB
Calculated MW 516kDa
Observed MW 250kDa/

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Mouse plasma, Rat plasma,
Cellular location Cytoplasm, Secreted
Customer validation

WB (Rattus norvegicus, Homo sapiens, Pelteobagrus fulvidraco)

Documents

Certificate of Compliance

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Lot number

Research Area

ApoB Rabbit pAb images

ABclonal:Western blot - ApoB Rabbit pAb (A1330)}

Western blot - ApoB Rabbit pAb (A1330)

Western blot analysis of various lysates using ApoB Rabbit pAb (A1330) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - ApoB Rabbit pAb (A1330)}

Immunofluorescence - ApoB Rabbit pAb (A1330)

Immunofluorescence analysis of mouse liver cells using ApoB Rabbit pAb (A1330) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on APOB. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to APOB. (Distance between topics and target gene indicate popularity.) APOB

* Data provided by citexs.com, for reference only.

Publishing research using A1330? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Proteins (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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