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ApoB Rabbit pAb (A1330)

Publications (2) Datasheet

ABclonal:Western blot - ApoB Rabbit pAb (A1330)

Western blot analysis of extracts of various cell lines, using ApoB antibody (A1330) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - ApoB Rabbit pAb (A1330)

Immunofluorescence analysis of C6 cells using ApoB antibody (A1330) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ApoB Rabbit pAb (A1330)

Immunofluorescence analysis of U-2 OS cells using ApoB antibody (A1330) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameApoB Rabbit pAb
Catalog No.A1330
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 28-330 of human ApoB (NP_000375.2).
SequenceEEEMLENVSLVCPKDATRFKHLRKYTYNYEAESSSGVPGTADSRSATRINCKVELEVPQLCSFILKTSQCTLKEVYGFNPEGKALLKKTKNSEEFAAAMSRYELKLAIPEGKQVFLYPEKDEPTYILNIKRGIISALLVPPETEEAKQVLFLDTVYGNCSTHFTVKTRKGNVATEISTERDLGQCDRFKPIRTGISPLALIKGMTRPLSTLISSSQSCQYTLDAKRKHVAEAICKEQHLFLPFSYKNKYGMVAQVTQTLKLEDTPKINSRFFGEGTKKMGLAFESTKSTSPPKQAEAVLKTLQ
Gene ID338
Swiss protP04114
SynonymsAPOB; FLDB; LDLCQ4; apoB-100; apoB-48
Calculated MW515kDa
Observed MW250KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesMouse plasma, Rat plasma
Cellular locationCytoplasm, Secreted
Customer validation

WB(Rattus norvegicus)

ABclonal:Western blot - ApoB Rabbit pAb (A1330) }

Western blot - ApoB Rabbit pAb (A1330)

Western blot analysis of extracts of various cell lines, using ApoB antibody (A1330) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - ApoB Rabbit pAb (A1330) }

Immunofluorescence - ApoB Rabbit pAb (A1330)

Immunofluorescence analysis of C6 cells using ApoB antibody (A1330) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ApoB Rabbit pAb (A1330) }

Immunofluorescence - ApoB Rabbit pAb (A1330)

Immunofluorescence analysis of U-2 OS cells using ApoB antibody (A1330) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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