Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Androgen Receptor Rabbit mAb (A19611)

PathoQIHC Pathology

Review (1) Publications (4) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Androgen Receptor Rabbit mAb (A19611)

Western blot analysis of lysates from mouse testis, using Androgen Receptor Rabbit mAb (A19611) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Androgen Receptor Rabbit mAb (A19611)

Immunohistochemistry analysis of Androgen Receptor in paraffin-embedded human prostate cancer using Androgen Receptor Rabbit mAb (A19611) at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Androgen Receptor Rabbit mAb (A19611)

Confocal imaging of paraffin-embedded Human prostate cancer using Androgen Receptor Rabbit mAb (A19611, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunoprecipitation - Androgen Receptor Rabbit mAb (A19611)

Immunoprecipitation analysis of 600 μg extracts of Mouse testis cells using 3 μg Androgen Receptor antibody (A19611). Western blot was performed from the immunoprecipitate using Androgen Receptor antibody (A19611) at a dilution of 1:1000.

Review
ABclonal: review for Androgen Receptor Rabbit mAb(A19611)

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Overview

Product name Androgen Receptor Rabbit mAb
Catalog No. A19611
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC0090

Background

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Androgen Receptor (P10275).
Sequence MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREVIQNPGPRHPEAASAAPPGASLLLLQQQQQQQQQQQQQQQQQQQQQQQETSPRQQQQQQGEDGSPQAH
Gene ID 367
Swiss prot P10275
Synonyms KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA; Androgen Receptor
Calculated MW 99kDa
Observed MW 110kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications Testing results
IHC-P Human
IF/ICC Human
WB MouseRat
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples Mouse testis
Cellular location Nucleus
Customer validation

(testicular tissue)

IF (Mus musculus, Homo sapiens)

WB (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Androgen Receptor Rabbit mAb images

ABclonal:Western blot - Androgen Receptor Rabbit mAb (A19611)}

Western blot - Androgen Receptor Rabbit mAb (A19611)

Western blot analysis of lysates from mouse testis, using Androgen Receptor Rabbit mAb (A19611) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Androgen Receptor Rabbit mAb (A19611)}

Immunohistochemistry - Androgen Receptor Rabbit mAb (A19611)

Immunohistochemistry analysis of Androgen Receptor in paraffin-embedded human prostate cancer using Androgen Receptor Rabbit mAb (A19611) at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Androgen Receptor Rabbit mAb (A19611)}

Immunofluorescence - Androgen Receptor Rabbit mAb (A19611)

Confocal imaging of paraffin-embedded Human prostate cancer using Androgen Receptor Rabbit mAb (A19611, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunoprecipitation - Androgen Receptor Rabbit mAb (A19611)}

Immunoprecipitation - Androgen Receptor Rabbit mAb (A19611)

Immunoprecipitation analysis of 600 μg extracts of Mouse testis cells using 3 μg Androgen Receptor antibody (A19611). Western blot was performed from the immunoprecipitate using Androgen Receptor antibody (A19611) at a dilution of 1:1000.

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Related Targets

Based on existing publications, the following genes are closely related to research on AR. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to AR. (Distance between topics and target gene indicate popularity.) AR

* Data provided by citexs.com, for reference only.

Publishing research using A19611? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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