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Androgen Receptor Rabbit pAb (A2053)

Reviews (2)Publications (5) Datasheet

ABclonal:Western blot - Androgen Receptor Polyclonal Antibody (A2053)

Western blot analysis of extracts of 22RV1 cells, using Androgen Receptor Antibody (A2053) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).

ABclonal:Immunohistochemistry - Androgen Receptor Polyclonal Antibody (A2053)

Immunohistochemistry of paraffin-embedded mouse brain using Androgen Receptor antibody (A2053) (40x lens).

Review
ABclonal: review for Androgen Receptor Rabbit pAb(A2053)
Review
ABclonal: review for Androgen Receptor Rabbit pAb(A2053)

Overview

Product nameAndrogen Receptor Rabbit pAb
Catalog No.A2053
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Androgen Receptor (NP_000035.2).
SequenceMEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREVIQNPGPRHPEAASAAPPGASLLLLQQQQQQQQQQQQQQQQQQQQQQQETSPRQQQQQQGEDGSPQAH
Gene ID367
Swiss protP10275
SynonymsAIS; AR8; DHTR; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM; AR
Calculated MW44kDa/67kDa/99kDa
Observed MW110kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples22Rv1
Cellular locationCytoplasm, Nucleus
Customer validation

(Mouse liver)

WB(Homo sapiens, Rattus norvegicus)

ABclonal:Western blot - Androgen Receptor Polyclonal Antibody (A2053) }

Western blot - Androgen Receptor Polyclonal Antibody (A2053)

Western blot analysis of extracts of 22RV1 cells, using Androgen Receptor Antibody (A2053) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
ABclonal:Immunohistochemistry - Androgen Receptor Polyclonal Antibody (A2053) }

Immunohistochemistry - Androgen Receptor Polyclonal Antibody (A2053)

Immunohistochemistry of paraffin-embedded mouse brain using Androgen Receptor antibody (A2053) (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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