Product Type > Antibodies > Secondary Antibodies

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS074)

Publications (3) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Wide range of species

ABclonal:Immunofluorescence - ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS074)

Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K9 Rabbit mAb (A22079) (40x lens), the secondary antibody was ABflo® 594-conjugated AffiniPure Goat Anti antibody (AS074) used at dilution of 1 : 200. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS074)

Immunofluorescence analysis of PC-12 cells using MonoMethyl-Histone H3-K9 Rabbit mAb (A22079) (40x lens) , the secondary antibody was ABflo® 594-conjugated AffiniPure Goat Anti antibody (AS074) used at dilution of 1 : 200. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS074)

Immunofluorescence analysis of HeLa cells using MonoMethyl-Histone H3-K9 Rabbit mAb (A22079) (40x lens) , the secondary antibody was ABflo® 594-conjugated AffiniPure Goat Anti antibody (AS074) at dilution of 1 : 200. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Flow CytoMetry - ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS074)

Flow cytometric analysis of Positive antibody E-Cadherin Rabbit mAb (2.5μg/mL) in various cells (orange) compare to Rabbit rabbit isotype control (blue) and non-staining control (Red).The secondary antibody used was ABflo® 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (AS074) at 1:100.

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Overview

Product name ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
Catalog No. AS074
Host species Goat
Purification method Affinity purification
Isotype ABflo™ 594 conjugated IgG

Background

Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.

Immunogen information

Immunogen Rabbit IgG
Sequence Email for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW

Applications

Reactivity Wide range of species
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • FC 1:100 - 1:800
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.025% Sodium Azide, 0.75% BSA, 50% glycerol, pH7.3.
Application key Flow Cytometry    Immunofluorescence    
Positive samples
Cellular location
Customer validation

IF (Homo sapiens, Mus musculus, Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) images

ABclonal:Immunofluorescence - ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS074)}

Immunofluorescence - ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS074)

Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K9 Rabbit mAb (A22079) (40x lens), the secondary antibody was ABflo® 594-conjugated AffiniPure Goat Anti antibody (AS074) used at dilution of 1 : 200. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS074)}

Immunofluorescence - ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS074)

Immunofluorescence analysis of PC-12 cells using MonoMethyl-Histone H3-K9 Rabbit mAb (A22079) (40x lens) , the secondary antibody was ABflo® 594-conjugated AffiniPure Goat Anti antibody (AS074) used at dilution of 1 : 200. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS074)}

Immunofluorescence - ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS074)

Immunofluorescence analysis of HeLa cells using MonoMethyl-Histone H3-K9 Rabbit mAb (A22079) (40x lens) , the secondary antibody was ABflo® 594-conjugated AffiniPure Goat Anti antibody (AS074) at dilution of 1 : 200. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Flow CytoMetry - ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS074)}

Flow CytoMetry - ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L) (AS074)

Flow cytometric analysis of Positive antibody E-Cadherin Rabbit mAb (2.5μg/mL) in various cells (orange) compare to Rabbit rabbit isotype control (blue) and non-staining control (Red).The secondary antibody used was ABflo® 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (AS074) at 1:100.

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Related Targets

Based on existing publications, the following genes are closely related to research on Goat Anti-Rabbit. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Goat Anti-Rabbit. (Distance between topics and target gene indicate popularity.) Goat Anti-Rabbit

* Data provided by citexs.com, for reference only.

Publishing research using AS074? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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