Western blot analysis of extracts of Jukat cells, using Active Caspase-3 Rabbit mAb (A11021) at 1:500 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
|Product name||Active Caspase-3 Rabbit mAb|
|Purification method||Affinity purification|
|Tested applications||WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP|
|Recommended dilution||WB 1:500 - 1:1000|
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.|
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
|Application key||Western blotting|
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* For research use only. Not for therapeutic or diagnostic purposes.