Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Activated Notch1 Rabbit mAb (A2349)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Activated Notch1 Rabbit mAb (A2349)

Western blot analysis of lysates from Molt-4 cells, using Activated Notch1 Rabbit mAb (A2349).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name Activated Notch1 Rabbit mAb
Catalog No. A2349
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma.

Immunogen information

Immunogen A synthetic peptide of human Activated Notch1
Sequence Email for sequence
Gene ID 4851
Swiss prot P46531
Synonyms hN1; AOS5; TAN1; AOVD1; Activated Notch1
Calculated MW 273kDa
Observed MW 126kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • FC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    Flow Cytometry    
Positive samples Molt-4
Cellular location Cell membrane, Nucleus, Single-pass type I membrane protein

Research Area

Activated Notch1 Rabbit mAb images

ABclonal:Western blot - Activated Notch1 Rabbit mAb (A2349)}

Western blot - Activated Notch1 Rabbit mAb (A2349)

Western blot analysis of lysates from Molt-4 cells, using Activated Notch1 Rabbit mAb (A2349).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on NOTCH1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to NOTCH1. (Distance between topics and target gene indicate popularity.) NOTCH1

* Data provided by citexs.com, for reference only.

Publishing research using A2349? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

ELISA Kits (1)

Proteins (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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