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β-Actin Rabbit mAb (High Dilution) (AC026)

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ABclonal:β-Actin Rabbit mAb (High Dilution)
ABclonal:Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)

Western blot analysis of extracts of various cell lines, using β-actin antibody (AC026) at 1:100000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)

Western blot analysis of extracts of HeLa cells, using β-actin antibody (AC026) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (High Dilution) (AC026)

Immunohistochemistry of paraffin-embedded human breast cancer using β-actin antibody (AC026) at dilution of 1:100 (20x lens).

ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (High Dilution) (AC026)

Immunohistochemistry of paraffin-embedded mouse liver using β-actin antibody (AC026) at dilution of 1:100 (20x lens).

ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (High Dilution) (AC026)

Immunohistochemistry of paraffin-embedded rat kidney using β-actin antibody (AC026) at dilution of 1:100 (20x lens).

Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)
Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)
Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)
Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)

Overview

Product nameβ-Actin Rabbit mAb (High Dilution)
Catalog No.AC026
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Note:Dilution method
Put 5ul AC026 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
ImmunogenRecombinant protein of human β-actin
SequenceEmail for sequence
Gene ID60
Swiss protP60709
SynonymsACTB; BRWS1; PS1TP5BP1; beta actin; actin
Calculated MW41kDa
Observed MW42KDa
ReactivityHuman, Mouse, Rat, Chicken, Zebrafish, Pig, Oryza sativa
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:50000 - 1:200000
IHC 1:200 - 1:2000
Antibody Dilute ProcedurePut 5ul AC026 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesTE-1, SW480, NIH/3T3, 293T, Mouse brain, Mouse testis, mose lung, rat brain
Cellular locationCytoplasm, cytoskeleton
Customer validation

(rat brain cells, Pig lung, 293T, DF-1, Mus musculus)

WB(Sus scrofa, Homo sapiens, Rattus norvegicus, Mus musculus, Squaliobarbus curriculus, Danio rerio, Alpinia katsumadai Hayata, Gallus gallus, Cyprinus carpio, Helicoverpa armigera, Anatinae, Cricetinae, Oreochroms mossambcus, Edwardsiella tarda, Drosophila melanogaster, Mauremys sinensis , Bombyx mori, Lotus japonicus, Ctenopharyngodon, Meretrix petechialis, Ctenopharyngodon idellus, Zea mays, Malus micromalus, Abalone, Ictalurus punctatus , Eospalax cansus, Mactra, Bos taurus, Cynoglossus semilaevis, Mycobacterium tuberculosis, Cavia porcellus, Macaca mulatta, Arabidopsis thaliana)

FC(Sus scrofa)

WB(Sus scrofa)

IF(Sus scrofa)

Co-IP(Alternaria alternata)

IP(Homo sapiens)

IP(Other)

IHC(Mus musculus)

(Mus musculus)

IHC(Mus musculus)

IP(Mus musculus)

ABclonal:Western blot - β-Actin Rabbit mAb (High Dilution) (AC026) }

Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)

Western blot analysis of extracts of various cell lines, using β-actin antibody (AC026) at 1:100000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - β-Actin Rabbit mAb (High Dilution) (AC026) }

Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)

Western blot analysis of extracts of HeLa cells, using β-actin antibody (AC026) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (High Dilution) (AC026) }

Immunohistochemistry - β-Actin Rabbit mAb (High Dilution) (AC026)

Immunohistochemistry of paraffin-embedded human breast cancer using β-actin antibody (AC026) at dilution of 1:100 (20x lens).
ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (High Dilution) (AC026) }

Immunohistochemistry - β-Actin Rabbit mAb (High Dilution) (AC026)

Immunohistochemistry of paraffin-embedded mouse liver using β-actin antibody (AC026) at dilution of 1:100 (20x lens).
ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (High Dilution) (AC026) }

Immunohistochemistry - β-Actin Rabbit mAb (High Dilution) (AC026)

Immunohistochemistry of paraffin-embedded rat kidney using β-actin antibody (AC026) at dilution of 1:100 (20x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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