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β-Actin Rabbit mAb (High Dilution) (AC026)

Reviews (4)Publications (701) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat, Chicken, Zebrafish, Pig

ABclonal:β-Actin Rabbit mAb (High Dilution)
ABclonal:Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)

Western blot analysis of extracts of various cell lines, using β-actin antibody (AC026) at 1:100000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)

Western blot analysis of extracts of HeLa cells, using β-actin antibody (AC026) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)
Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)
Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)
Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)

Overview

Product nameβ-Actin Rabbit mAb (High Dilution)
Catalog No.AC026
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Note：Dilution method
Put 5ul AC026 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
ImmunogenRecombinant protein of human β-actin
SequenceEmail for sequence
Gene ID60
Swiss protP60709
SynonymsACTB; BRWS1; PS1TP5BP1; beta actin; actin
Calculated MW41kDa
Observed MW42KDa
ReactivityHuman, Mouse, Rat, Chicken, Zebrafish, Pig
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:50000 - 1:200000
Antibody Dilute ProcedurePut 5ul AC026 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesTE-1, SW480, NIH/3T3, 293T, Mouse brain, Mouse testis, mose lung, rat brain
Cellular locationCytoplasm, cytoskeleton
Customer validation

(rat brain cells, Pig lung, 293T, DF-1)

WB(Mus musculus, Rattus norvegicus, Mactra, Gallus gallus, Homo sapiens, Bos taurus, Sus scrofa, Danio rerio, Zea mays, Cynoglossus semilaevis, Mycobacterium tuberculosis, Cavia porcellus, Macaca mulatta, Helicoverpa armigera, Arabidopsis thaliana, Squaliobarbus curriculus, Malus micromalus, Cyprinus carpio, Alpinia katsumadai Hayata, Anatinae, Oreochroms mossambcus, Cricetinae, Edwardsiella tarda, Drosophila melanogaster, Mauremys sinensis , Bombyx mori, Ictalurus punctatus , Lotus japonicus, Meretrix petechialis, Ctenopharyngodon, Eospalax cansus, Cotton bollworms, Ctenopharyngodon idellus, broiler embryos, Flounder, Echinococcus multilocularis, Nicotiana benthamiana, Vaccinium dunalianum, Caenorhabditis elegans, Common carp, Escherichia coli, Hordeum vulgare, Haliotis, turtle, Eleusine indica, Danio rerio , Solanum lycopersicum)

IHC(Mus musculus, Homo sapiens)

IHC(Mus musculus, Homo sapiens)

IP(Homo sapiens)

IP(Mus musculus, Homo sapiens)

IHC(Mus musculus)

FC(Sus scrofa)

WB(Sus scrofa, Homo sapiens)

IF(Sus scrofa)

Co-IP(Alternaria alternata)

IP(Other, Danio rerio, Homo sapiens)

Co-IP(Mus musculus)

IF(Rattus norvegicus)

IF(Mus musculus, Homo sapiens)

ABclonal:Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)}

Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)

Western blot analysis of extracts of various cell lines, using β-actin antibody (AC026) at 1:100000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)}

Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)

Western blot analysis of extracts of HeLa cells, using β-actin antibody (AC026) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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