Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-p53-K382 Rabbit mAb (A16324)

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - Acetyl-p53-K382 Rabbit mAb (A16324)

Western blot analysis of extracts of various cell lines, using Acetyl-p53-K382 antibody (A16324) at 1:1000 dilution.HeLa cells and NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Overview

Product name	Acetyl-p53-K382 Rabbit mAb
Catalog No.	A16324
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Immunogen information

Immunogen	A synthetic acetylated peptide around K382 of human p53 (NP_000537.3).
Sequence	KKGQSTSRHKKLMFKTEGPDS
Gene ID	7157
Swiss prot	P04637
Synonyms	P53; BCC7; LFS1; BMFS5; TRP53; Acetyl-p53-K382
Calculated MW	44kDa
Observed MW	53kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key	Western blotting
Positive samples	HeLa TSA, NIH/3T3 TSA
Cellular location	Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Customer validation	WB (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

Acetyl-p53-K382 Rabbit mAb images

Western blot - Acetyl-p53-K382 Rabbit mAb (A16324)

Product Protocols

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Related Targets

Based on existing publications, the following genes are closely related to research on TP53. (Numbers indicate number of published articles where both targets appear.)

◀1/▶

Related Research Area

Based on existing publications, the following research topics are related to TP53. (Distance between topics and target gene indicate popularity.) TP53

* Data provided by citexs.com, for reference only.

References for Acetyl-p53-K382 Rabbit mAb (A16324)

Product:

Acetyl-p53-K382 Rabbit mAb

Journal:

Mol Med Rep

Application:

IF:

2.952

Species:

Homo sapiens

PMID:

33576462

Title:

MicroRNA‑138‑5p drives the progression of heart failure via inhibiting sirtuin 1 signaling

References for Acetyl-p53-K382 Rabbit mAb (A16324)

Product:

Acetyl-p53-K382 Rabbit mAb

Journal:

Toxicol Appl Pharmacol

Application:

IF:

4.219

Species:

Homo sapiens

PMID:

34798143

Title:

Up-regulation of PUMA caused the activation of p53 phosphorylation and acetylation, enhancing the interaction between PUMA and Bcl-X and mediating arsenic …

References for Acetyl-p53-K382 Rabbit mAb (A16324)

Product:

Acetyl-p53-K382 Rabbit mAb

Journal:

Redox biology

Application:

IF:

11.79

Species:

Homo sapiens

PMID:

35636016

Title:

Inhibition of nuclear deacetylase Sirtuin-1 induces mitochondrial acetylation and calcium overload leading to cell death

References for Acetyl-p53-K382 Rabbit mAb (A16324)

Product:

Acetyl-p53-K382 Rabbit mAb

Journal:

Molecular metabolism

Application:

IF:

8.56

Species:

Mus musculus

PMID:

35987498

Title:

Pgc-1α controls epidermal stem cell fate and skin repair by sustaining NAD+ homeostasis during aging

References for Acetyl-p53-K382 Rabbit mAb (A16324)

Product:

Acetyl-p53-K382 Rabbit mAb

Journal:

Evidence-based complementary and alternative medicine : eCAM

Application:

IF:

2.65

Species:

Homo sapiens

PMID:

35911149

Title:

Arisaema heterophyllum Blume Monomer Stigmasterol Targets PPARγ and Inhibits the Viability and Tumorigenicity of Lung Adenocarcinoma Cells NCI-H1975 - PMC