Western blot analysis of extracts of various cell lines, using Acetyl-p53-K382 antibody (A16324) at 1:1000 dilution.HeLa cells and NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
|Product name||Acetyl-p53-K382 Rabbit mAb|
|Purification method||Affinity purification|
|Tested applications||WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition|
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
|Application key||Western blotting|
|Positive samples||HeLa TSA, NIH/3T3 TSA|
|Cellular location||Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body|
WB (Homo sapiens, Mus musculus)
To download a Certificate of Compliance, please enter your Lot number below:
Submit your question about A16324 below and we will get back to you within one business day.
Alternatively, call us at 888.754.5670.
Based on existing publications, the following genes are closely related to research on TP53. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to TP53. (Distance between topics and target gene indicate popularity.) TP53
* Data provided by citexs.com, for reference only.
Publishing research using A16324? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.