Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H4-K91 Rabbit pAb (A7409)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range)

ABclonal:Western blot - Acetyl-Histone H4-K91 Rabbit pAb (A7409)

Western blot analysis of extracts of NIH/3T3 cells, using Acetyl-Histone H4-K91 antibody (A7409) at 1:1000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 300s.

ABclonal:Immunofluorescence - Acetyl-Histone H4-K91 Rabbit pAb (A7409)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K91 antibody (A7409) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H4-K91 Rabbit pAb (A7409)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H4-K91 antibody (A7409) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H4-K91 Rabbit pAb (A7409)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K91 antibody (A7409) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

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Overview

Product name Acetyl-Histone H4-K91 Rabbit pAb
Catalog No. A7409
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Immunogen information

Immunogen A synthetic acetylated peptide around K91 of human Histone H4 (NP_003529.1).
Sequence ALKRQ
Gene ID 8359
Swiss prot P62805
Synonyms H4; H4/n; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4F2; H4FN; FO108; H4-16; H4C11; H4C12; H4C13; H4C15; H4C16; HIST2H4; HIST2H4A; Acetyl-Histone H4-K91
Calculated MW 11kDa
Observed MW 17kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples NIH/3T3
Cellular location Chromosome, Nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

Acetyl-Histone H4-K91 Rabbit pAb images

ABclonal:Western blot - Acetyl-Histone H4-K91 Rabbit pAb (A7409)}

Western blot - Acetyl-Histone H4-K91 Rabbit pAb (A7409)

Western blot analysis of extracts of NIH/3T3 cells, using Acetyl-Histone H4-K91 antibody (A7409) at 1:1000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 300s.
ABclonal:Immunofluorescence - Acetyl-Histone H4-K91 Rabbit pAb (A7409)}

Immunofluorescence - Acetyl-Histone H4-K91 Rabbit pAb (A7409)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K91 antibody (A7409) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H4-K91 Rabbit pAb (A7409)}

Immunofluorescence - Acetyl-Histone H4-K91 Rabbit pAb (A7409)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H4-K91 antibody (A7409) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H4-K91 Rabbit pAb (A7409)}

Immunofluorescence - Acetyl-Histone H4-K91 Rabbit pAb (A7409)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K91 antibody (A7409) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on H4C14. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H4C14. (Distance between topics and target gene indicate popularity.) H4C14

* Data provided by citexs.com, for reference only.

Publishing research using A7409? Please let us know so that we can cite the reference in this datasheet.

Antibodies (32)

Secondary Antibodies (22)

* For research use only. Not for therapeutic or diagnostic purposes.

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