Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H4-K16 Rabbit pAb (A5280)

Publications (2) Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat, Other (Wide Range)

ABclonal:Western blot - Acetyl-Histone H4-K16 Rabbit pAb (A5280)

Western blot analysis of various lysates, using Acetyl-HistoneH4-K16 antibody (A5280) at 1:400 dilution.NIH/3T3 and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - Acetyl-Histone H4-K16 Rabbit pAb (A5280)

Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H4-K16 Rabbit pAb (red, A5280) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H4-K16 Rabbit pAb (A5280)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K16 (A5280) at dilution of 1:100. Blue: DAPI for nuclear staining.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H4-K16 Rabbit pAb (A5280)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K16 (A5280) at dilution of 1:100. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H4-K16 Rabbit pAb (A5280)

Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H4-K16 (A5280) at dilution of 1:100. Blue: DAPI for nuclear staining.U2OS cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

Overview

Product nameAcetyl-Histone H4-K16 Rabbit pAb
Catalog No.A5280
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Immunogen information

ImmunogenA synthetic acetylated peptide around K16 of human Histone H4 (NP_001029249.1).
SequenceGGAKR
Gene ID8370
Swiss protP62805
SynonymsFO108; H4; H4/n; H4F2; H4FN; HIST2H4; Histone H4; HIST1H4A; HIST2H4A
Calculated MW11kDa
Observed MW11KDa

Applications

ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyWestern blotting    
Positive samplesNIH/3T3, C6
Cellular locationChromosome, Nucleus
Customer validation

WB(Frog, Mus musculus)

Research Area

Acetyl-Histone H4-K16 Rabbit pAb images

ABclonal:Western blot - Acetyl-Histone H4-K16 Rabbit pAb (A5280)}

Western blot - Acetyl-Histone H4-K16 Rabbit pAb (A5280)

Western blot analysis of various lysates, using Acetyl-HistoneH4-K16 antibody (A5280) at 1:400 dilution.NIH/3T3 and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - Acetyl-Histone H4-K16 Rabbit pAb (A5280)}

Immunofluorescence - Acetyl-Histone H4-K16 Rabbit pAb (A5280)

Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H4-K16 Rabbit pAb (red, A5280) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H4-K16 Rabbit pAb (A5280)}

Immunofluorescence - Acetyl-Histone H4-K16 Rabbit pAb (A5280)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K16 (A5280) at dilution of 1:100. Blue: DAPI for nuclear staining.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H4-K16 Rabbit pAb (A5280)}

Immunofluorescence - Acetyl-Histone H4-K16 Rabbit pAb (A5280)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K16 (A5280) at dilution of 1:100. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H4-K16 Rabbit pAb (A5280)}

Immunofluorescence - Acetyl-Histone H4-K16 Rabbit pAb (A5280)

Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H4-K16 (A5280) at dilution of 1:100. Blue: DAPI for nuclear staining.U2OS cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on Histone H4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Histone H4. (Distance between topics and target gene indicate popularity.) Histone H4

* Data provided by citexs.com, for reference only.

Publishing research using A5280? Please let us know so that we can cite the reference in this datasheet.

Primary Antibodies (20)

Secondary Antibodies (26)

* For research use only. Not for therapeutic or diagnostic purposes.

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