Reactivity:Human, Mouse, Rat, Other (Wide Range)
Western blot analysis of various lysates, using Acetyl-HistoneH4-K16 antibody (A5280) at 1:400 dilution.NIH/3T3 and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H4-K16 Rabbit pAb (red, A5280) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K16 (A5280) at dilution of 1:100. Blue: DAPI for nuclear staining.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K16 (A5280) at dilution of 1:100. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
|Product name||Acetyl-Histone H4-K16 Rabbit pAb|
|Purification method||Affinity purification|
|Immunogen||A synthetic acetylated peptide around K16 of human Histone H4 (NP_001029249.1).|
|Synonyms||H4; H4/n; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4F2; H4FN; FO108; H4-16; H4C11; H4C12; H4C13; H4C15; H4C16; HIST2H4; HIST2H4A; Acetyl-Histone H4-K16|
|Reactivity||Human, Mouse, Rat, Other (Wide Range)|
|Tested applications||WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition|
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
|Application key||Western blotting Immunofluorescence|
|Positive samples||NIH/3T3, C6|
|Cellular location||Chromosome, Nucleus|
WB (Frog, Mus musculus, Nicotiana benthamiana, Homo sapiens)
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Based on existing publications, the following genes are closely related to research on H4C14. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to H4C14. (Distance between topics and target gene indicate popularity.) H4C14
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* For research use only. Not for therapeutic or diagnostic purposes.